摘要
目的:从不同产地和不同批次的原料药材中筛选出较优的原料药材,并建立芪斛楂颗粒的HPLC指纹图谱。方法:参考《中国药典》2015版及相关文献对陈皮、甘草、木瓜、等11味处方原药材进行成分检测和筛选,采用HPLC特征图谱技术,建立芪斛楂颗粒的指纹图谱分析方法,采用国家药典委员会中药色谱指纹图谱相似度评价系统软件(2012版)进行相似度分析。结果:通过对芪斛楂颗粒指标成分含量测定结果,筛选出较优的药材来源。芪斛楂颗粒的HPLC指纹图谱共确定了16个共有峰,指纹图谱相似度均在0.9以上,并指认出2个共有成分(甘草苷,橙皮苷)。结论:所建立的方法简便、重复性好、稳定性好,可为芪斛楂颗粒的质量标准提升提供依据。
Objective This subject selects the better raw medicinal materials from different origin different batches of raw materials.To establish the HPLC fingerprint of Qihuzha Granules.Methods Reference the“Chinese Pharmacopoeia”in the 2015 edition and the literature methods,the content of active ingredients in citri reticulatae pericarpium,glycyrrhizae radix et rhizoma,chaenomelis fructus,ete,have been measured.The HPLC characteristic spectrum technology was usedto analyze the characteristic spectrum ofQihuzha Granules.The similarity ofthe species wasevaluated by using software“Similarity Evaluation System for Chromatographic Fingerprint of TCM”published by the State Pharmacopoeia Commission(2012A version).ResultsIt has selected better medicines source through the results of measuring the content of the target composition.There were 16 common peaks in the HPLC chromatographic fingerprint of Qihuzha Granules,and 2 common components(Liquiritin,hesperidin)were identified.The similar degrees of 10 batches of samples were over 0.900.Conclusion This method is simple,repeatable,and stable.It provides basic data for improving the standand of Qihuzha Granules.
作者
安巧
冯敏
肖正会
万科
柳小兰
王海波
王道平
AN Qiao;FENG Min;XIAO Zhenghui;WAN Ke;LIU Xiaolan;WANG Haibo;WANG Daoping(State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550014,China;The Key laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;Guizhou Medical University,Guiyang 550025,China;Sunflower Pharmaceutical Group(Guizhou)Hongqi Co.,Ltd.,Liupanshui 550000,China)
出处
《中国民族民间医药》
2021年第19期40-45,共6页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
贵州省科技计划项目:贵州省中国科学院天然产物化学重点实验室信息化建设(黔科合平台人才[2017]5713)
贵阳市科技项目:药物技术服务平台及创新能力建设(筑科合同[2017]8-2号)
贵阳市高新区科技项目:贵州省中药民族药技术创新共享服务平台建设(GXCX-2018-001)。