基于生物信息学分析的激素性股骨头坏死相关核心基因和通路识别

Identification of Core Genes and Pathways Related to Steroid-induced Osteonecrosis of the Femoral Head Based on Bioinformatics Analysis

目的对激素性股骨头坏死(SONFH)差异表达基因进行生物信息学分析,确定与SONFH的发生和发展相关的关键致病生物标志物,并预测潜在的SONFH治疗药物。方法从基因表达总表(GEO)数据库中下载GSE123568表达谱,采用GEO2R在线工具软件分析,获得差异表达基因(DGEs)。通过String数据库构建SONFH差异表达基因蛋白质相互作用(PPI)网络,通过Cytoscape软件cytoHubba插件筛选出核心基因,并进行差异表达基因功能富集分析、通路富集分析和基因富集分析(GSEA),SONFH潜在治疗药物的预测。结果共筛选出了160个SONFH差异表达基因,在各种关键途径中高度富集,包括红细胞发展、JAK-STAT通路、toll受体通路等。DGIdb数据库中预测了3个潜在治疗药物,分别是甘氨酸、富马酸亚铁、硫酸甘氨酸亚铁。结论研究确定了10个潜在基因,可能成为早期筛查的重要标志物;甘氨酸、富马酸亚铁、硫酸甘氨酸亚铁是SONFH潜在的治疗药物。

Objective Bioinformatics analysis of differentially expressed genes in steroid-induced osteonecrosis of the femoral head(SONFH)was performed to identify key pathogenic biomarkers associated with the occurrence and development of SONFH,and predict potential SONFH therapies.Methods The GSE123568 Expression profile was downloaded from the Gene Expression Omnibus(GEO)database,the differentially expressed genes(DGEs)were analyzed using the GEO2R online tool.SONFH differentially expressed gene protein interaction(PPI)network was constructed through String database,core genes were screened through cytoHubba of Cytoscape software.And functional enrichment analysis,pathway enrichment analysis and prediction of SONFH potential therapeutic drugs were performed.Results A total of 160 differentially expressed SONFH genes were selected,which were highly enriched in various key pathways,including erythrocyte development,JAK-stat pathway and toll receptor pathway.The DGIdb database was predicted three potential therapeutic agents,namely ferrous glycine,ferrous fumarate,and ferrous glycine sulfate.Conclusion The study identified for the first time 10 potential genes that could be important markers for early screening.Glycine,ferrous fumarate and ferrous sulfate are potential therapeutic agents for SONFH.