自噬相关蛋白Atg5和Beclin-1在宫颈癌组织中的表达及与临床病理的相关性

The Expression of Autophagy-related Proteins Atg5 and Beclin-1 in Cervical Cancer Tissues and Their Correlation with Clinicopathology

目的探讨自噬相关蛋白Atg5和Beclin-1在宫颈癌组织中的表达及与临床病理的相关性。方法选取60例宫颈癌患者作为研究对象,将其分为恶性组,取同期60例良性子宫肌瘤或子宫腺肌瘤行全子宫切除手术的患者作为良性组,另取同期60例健康者的子宫颈组织标本进行分析,将其分为对照组。分析三组患者自噬相关基因5及Beclin1两种蛋白的表达情况、阳性率以及临床病理情况;应用回归分析,分析自噬相关基因5及Beclin1两种蛋白与宫颈癌病理的相关性。结果三组患者Atg5 mRNA及Beclin-1 mRNA表达情况对比差异显著,恶性组明显低于良性组和对照组(P<0.05);三组患者Atg5阳性与阴性患者例数对比差异显著,Beclin1阳性与阴性患者例数对比差异显著(P<0.05);60例宫颈癌患者中,Atg5阳性与Atg5阴性患者月经状态、年龄、肿瘤大小对比无明显差异(P>0.05),两组患者的临床分期、淋巴结转移、组织分化程度对比差异显著(P<0.05),Beclin1阳性与Beclin1阴性患者月经状态、年龄、肿瘤大小对比无明显差异(P>0.05),两组患者的临床分期、淋巴结转移、组织分化程度对比差异显著(P<0.05);Spearman相关分析显示,月经状态、年龄、肿瘤大小与自噬相关基因Beclin1和Atg5不相关(P>0.05),临床分期、淋巴结转移和组织分化程度与自噬相关基因Beclin1和Atg5呈负相关(P<0.05)。结论自噬相关基因5(Atg5)及Beclin-1两种蛋白在宫颈癌患者的癌组织中表达低于良性肿瘤患者和健康人士,随着患者病情加重,自噬相关基因5(Atg5)及Beclin-1两种蛋白表达明显降低,自噬相关基因5(Atg5)及Beclin-1与宫颈癌患者的临床分期、淋巴结转移和组织分化程度呈负相关,因此可以应用自噬相关基因5(Atg5)及Beclin1来判断宫颈癌患者病情的严重程度。

Objective To investigate the expression of of autophagy-related proteins Atg5 and Beclin-1 in cervical cancer tissues and their correlation with clinicopathology.Methods 60 cases of patients with cervical cancer were selected as malignant group,60 cases of benign uterine fibroids or adenomyoma patients underwent total hysterectomy in the same period were selected as the benign group,and the cervical tissue samples of 60 healthy people in the same period were the control group.The expression,positive rate and clinicopathological conditions of autophagy-related gene 5 and Beclin1 proteins in the 3 groups were analyzed.The correlation between autophagy related gene 5 and Beclin1 protein and cervical cancer pathology was analyzed by regression analysis.Results The expressions of Atg5 mRNA and Beclin-1 mRNA in the 3 groups were significantly different,and the malignant group was significantly lower than that of the benign group and the control group(P<0.05);There were significant differences in the number of Atg5 positive and negative patients in the 3 groups,and the number of Beclin1 positive and negative patients was significantly different(P<0.05);In 60 cases of cervical cancer,there was no significant difference in menstrual state,age and tumor size between ATG5 positive and ATG5 negative patients.There were significant differences in clinical stage,lymph node metastasis and tissue differentiation between the 2 groups(P<0.05).There was no significant difference in menstrual state,age and tumor size between Beclin1 positive and Beclin1 negative patients(P>0.05).There were significant differences in clinical stage,lymph node metastasis and tissue differentiation between the 2 groups(P<0.05);Spearman correlation showed that menstrual status,age and tumor size were not correlated with autophagy related genes Beclin1 and ATG5(P>0.05),while clinical stage,lymph node metastasis and tissue differentiation were negatively correlated with autophagy related genes Beclin1 andATG5(P<0.05).Conclusion The expression of ATG5 and beclin-1 in cervical cancer is lower than that in benign tumor and healthy people.The expression of ATG5 and beclin-1 is significantly lower in patients with cervical cancer than in patients with benign tumor and healthy people.The expression of ATG5 and beclin-1 in patients with cervical cancer is significantly lower than that in patients with cervical cancer Therefore,autophagy related gene 5(ATG5)and Beclin1 can be used to determine the severity of cervical cancer.