不同品种猪背最长肌中IGF-1-PI3K/AKT通路基因的生长发育表达模式差异

Differential Expression Patterns ofGrowth and Development of IGF-1-PI3K/AKT Pathway Genes in Longissimus Dorsi of Different Pig Breeds

本文研究了广西巴马小型猪和长白猪背最长肌中IGF-1-PI3K/AKT信号通路相关基因的发育表达模式的差异,为今后研究巴马小型猪的生长发育和肉质性状提供分子理论基础。克隆猪INSR、IRS1、IGF-1、PI3K、PI3KR1、PDK1、PTEN、AKT1、FoxO1、mTOR、4EBP1和β-actin基因,构建荧光定量PCR标准曲线,采用实时PCR法检测上述基因在广西巴马小型猪和长白猪出生后1、30、60、90、180 d背最长肌的表达变化。在同一生长发育阶段,PTEN和4EBP1基因的表达均极显著高于同一品种其他基因的表达,FoxO1、PI3KR1和AKT1基因中度表达,IGF-1、INSR、IRS1、PI3K、PDK1基因低度表达;在不同生长发育阶段基因的表达存在显著差异;两个品种猪在同一生长发育时间的表达也存在差异。本研究结果表明广西巴马小型猪和长白猪IGF-1-PI3K/AKT通路相关基因的表达呈现出明显的品种差异性和时空差异性,PTEN、FoxO1和4EBP1基因表达差异可能是两个品种猪肌肉产量和肉质性状存在差异的原因之一。

The differential expression patterns of growth and development of IGF-1-PI3K/AKT pathway in longissimus dorsi muscles of Guangxi Bama mini-pig and Landrace were studied in this paper,providing molecular theoretical basis for future research on the growth and meat quality of Guangxi Bama mini-pig.Porcine INSR,IRS1,IGF-1,PI3K,PI3KR1,PDK1,PTEN,AKT1,FoxO1,mTOR,4EBP1 andβ-actin genes of pig were cloned and the fluorescence quantitative PCR standard curve was constructed.The expression of above genes in the longest longissimus dorsi muscles of Guangxi Bama mini-pigs and Landrace pigs at 1,30,60,90 and 180 days were detected by Real-time PCR.At the same growth and development stage,the expression of PTEN and 4EBP1 genes was significantly higher than that of other genes in the same pig species,FoxO1,PI3KR1 and AKT1 geneswere moderately expressed,IGF-1,INSR,IRS1,PI3K and PDK1 genes were lowly expressed.There were significant differences in gene expression at different growth and development stages,and there were differences in gene expression at the same growth and development time between the two breeds.The expression patterns of IGF-1-PI3K/AKT pathway genes in Guangxi Bama mini-pig and Landrace showed significantly differences in breeds and in space-time,PTEN,FoxO1 and 4EBP1 gene may be one of the reasons for the differences in meat production and meat quality traits between the two breeds of pigs.