牦牛miR-383的靶基因预测及生物信息学分析

Target Gene Prediction and Bioinformatics Analysis of Yak miR-383

MicroRNAs(miRNAs)是一类非编码的小分子单链RNA,通过与靶基因的mRNA结合,抑制mRNA的翻译,参与多种生物学过程。本实验室前期通过高通量测序发现90日龄牦牛胚胎的背最长肌中miR-383的表达量显著高于成年牦牛。为探究miR-383在牦牛骨骼肌发育中的分子功能和机制,本研究对miR-383的靶基因进行预测,并进行生物信息学分析。通过TargetScan、miRDB和miRanda 3个软件预测了miR-383的靶基因,然后合并miRTarbase数据库中已被证实的靶基因作为基因集,分别用DAVID和KOBAS3.0在线软件对基因集进行功能注释(GO分析)和Pathway信号通路富集分析。结果表明,牦牛miR-383序列在各物种间高度保守,靶基因功能富集于CD8阳性T细胞增殖的调控、核糖核蛋白复合物定位和负调控T细胞分化等生物学过程。信号通路分析发现靶基因的信号通路显著富集于PI3K-Ak、AMPK、FoxO和Focal adhesion等与肌肉发育相关的信号通路中。该研究结果将为miR-383功能及调控机制的深入研究提供参考依据,也为解析牦牛肌肉发育的分子机制提供新的研究方向。

MicroRNAs(miRNAs)are a class of small non-coding single-stranded RNAs that are involved in a variety of biological processes by binding to the mRNA of target genes,inhibiting the translation of mRNA.High throughput sequencing results showed that the expression level of miR-383 in the longissimus dorsi muscle of embryo at 90 days old was significantly higher than that of adult yak.In order to explore the molecular function and mechanism of miR-383 in the development of yak skeletal muscle,we predicted the target genes of miR-383 and analyzed them with relative bioinformatics methods.The target genes of miR-383 were predicted by using the TargetScan,miRDB and miRanda software and the intersection of the results combined with the validated target gene from miRTarbase database as gene set for further Gene Ontology and KEGG pathway analysis.The results showed that the sequence of yak miR-383 was conserved among various species.The target genes of miR-383 were involved in multiple biological processes,such as regulation of CD8-positive,alpha-beta T cell proliferation,ribonucleoprotein complex localization,negative regulation of regulatory T cell differentiation,etc.The signaling pathway analysis suggested that the target genes were significantly enriched in muscle development related pathways,such as PI3K-Akt,AMPK,FoxO and Focal adhesion signaling pathway,etc.These results will provide a reference for the mechanism research of miR-383 and novel direction for the molecular mechanism study of muscle development in yaks.