海南岛中南部热带雨林杀秀丽隐杆线虫Bt菌株筛选

Screening of Bt Strains with Toxicity to Caenorhabditis elegans from Tropical Rain Forest in Central and Southern Hainan Island

植物寄生线虫对全世界的农作物造成了严重破坏并且难以防治,迫切需要开发高效并对环境友好的生物防治策略。本研究从海南中南部的尖峰岭、五指山、大里山、鹦哥岭热带雨林共采集1613份土壤样品,筛选分离出芽孢杆菌4399份,鉴定出Bt分离株224株,Bt菌株的分离率为5.09%。以模式生物秀丽隐杆线虫作为生物测定的靶标,筛选鉴定6株Bt菌株芽孢期蛋白对秀丽隐杆线虫有较好的杀虫活性。本研究在国内是首次对海南岛热带雨林杀线虫细菌资源进行调查,获得的杀线虫Bt菌株将来有可能进一步应用于植物线虫的生物防治。

Plant parasitic nematode has caused severe damage to crops around the world and is difficult to control.It is urgent to develop efficient and environmentally friendly strategy such as biological control.In this study,we collected 1613 soil samples from the tropical rain forests of Jianfengling,Wuzhishan,Dalishan,and Yinggeling in the central and southern Hainan.4399 Bacillus were screened from the soil samples,from which the 224 Bt were identified using microscope observation.The isolation rate of Bt strain reached 5.09%.The model organism Caenorhabditis elegans acted as the target insect for bioassay.We detected 6 Bt isolates,whose parasporal crystal protein exhibited good insecticidal activity against C.elegans larva.To our knowledge,this is the first report that described the nematicidal Bt resource from the tropical rainforest of Hainan Island in china.The nematicidal Bt strain and gene could be further applied to the biological control of plant nematodes in the future.