基于转录组分析酸枣仁斯皮诺素生物合成途径的相关基因

Identification of Genes Related to Biosynthesis of Spinosin in Ziziphi spinosae semen by Transcriptome Sequence Analysis

采用转录组测序技术分析酸枣仁(Ziziphi spinosae semen)不同发育时期基因表达差异,识别酸枣仁黄酮生物合成途径的相关基因,为进一步分析酸枣仁中黄酮类成分生物合成相关酶基因提供研究基础。以3个不同生长发育时期的酸枣仁样本为供试材料,采用Illumina Hiseq 4000进行转录组测序,并从头拼接,对转录本进行功能注释和差异性分析。经StringTie软件组装拼接得到74472个转录本,其中有26.83%在NR、Swiss-Prot、Pfam、GO、KOG、KEGG数据库中得到注释;代谢通路分析结果发现,在酸枣仁转录组中与黄酮类合成途径相关的11条差异表达基因可能编码斯皮诺素合成途径的一些酶类。酸枣仁中黄酮类成分斯皮诺素相关转录本信息为阐明斯皮诺素在生长期的积累提供重要依据,为提高酸枣仁品质研究提供参考。

The transcriptome sequencing technology was used to analyze the difference of gene expression in different developmental stages of Ziziphi spinosae semen,and the related genes of the biosynthesis pathway of flavonoids in Ziziphi spinosae semen were identified,which laid a foundation for further analysis of the enzymes related to biosynthesis of flavonoids.Three samples of Ziziphi spinosae semen at different growth and development stages were used as test materials.Illumina Hiseq 4000 was used for transcriptome sequencing,and the transcripts were functionally annotated and differentially analyzed.A total of 74472 transcripts were obtained by StringTie software assembly,of which 26.83%were annotated in NR,Swiss-Prot,Pfam,GO,KOG,KEGG databases.Metabolic pathway analysis found that flavonoids in the transcriptome of Ziziphi spinosae semen the 11 differen tially expressed genes associated with the synthetic pathway may encode some of the enzymes of the spinosin synthesis pathway.The transcript information of flavonoids in Ziziphi spinosae semen provided an important basis for clarifying the accumulation of spinosin in the growth phase,and a reference for improving the quality of it.