宏基因组测序法研究管材对生物膜致病菌的影响

Effects of pipe materials on pathogens in biofilms by metagenomic sequencing

为研究供水管道材料对管道生物膜致病菌种的影响,以已稳定运行8年的中国东部某城市实际供水管道中试平台管道为实验对象,从平行设置的球墨铸铁管(DCIP)、高密度聚乙烯管(HDPE)、镀锌钢管(GSP)、不锈钢复合管(SSCP)和钢管(SP)管道中收集13组生物膜样品,开展宏基因组测序分析,对比研究不同管道材料的致病菌种类分布情况。结果表明:DCIP生物膜中致病菌相对含量最为丰富,GSP致病菌相对含量最少;此外,世界卫生组织颁布的12种全球优先致病菌(priority pathogens)在本次供水管道中发现9种,在不同管道的相对丰度为0.079%~1.084%,表明供水管道生物膜对人类和环境的严重危害潜力;依据致病菌在5种管材中相对丰度的分布情况,发现与5种管材相关联的优势致病菌。研究结果为基于致病风险控制的供水管道管材选择提供了理论依据。

To investigate the influence of pipe materials on species of pathogens in water distribution system,a drinking water distribution system(DWDS)in a city in eastern China which has been operating steadily for eight years was taken as the experimental object.Metagenomic sequencing analysis was conducted to study the effects of pipeline materials on pathogens in 13 sets of biofilms collected from ductile iron pipe(DCIP),polyethylene pipe(HDPE),galvanized steel pipe(GSP),stainless steel composite pipe(SSCP),and steel pipe(SP).Results show that the relative abundance of pathogens in DCIP biofilm was the most,while that in GSP biofilm was the least.In addition,nine of 12 pathogens on the global priority pathogens list published by WHO were detected from the water distribution pipeline,with relative abundance ranged from 0.079%to 1.084%,indicating the severe hazard potential of biofilms in water supply pipelines to humans and the environment.Based on the relative abundance distributions of the pathogens in five pipe materials,the priority pathogens associated with the five pipe materials were found.The research results provide an effective and theoretical basis for the selection of water distribution pipe materials.