夏枯草对胶质瘤U87细胞凋亡与增殖的影响

Effects of Prunella Vulgaris on Apoptosis and Proliferation of Glioma Cell Line U87

目的:探讨夏枯草对胶质瘤U87细胞增殖凋亡的作用及机制。方法:检测对照组、夏枯草低、中和高剂量组细胞增殖,毒性,生长,凋亡,Bax、Bcl-2mRNA及蛋白,Caspase-3、Caspase-9 mRNA,Cleaved-Caspase-3、Cleaved-Caspase-9。制备U87细胞负瘤小鼠,分为模型组、夏枯草低、中和高剂量组,检测各组小鼠皮下瘤体积、增殖指数。结果:与对照组比较,夏枯草组细胞集落数量、细胞活性,Bcl-2 mRNA及蛋白均降低,凋亡细胞数,Bax、Caspase-3、Caspase-9mRNA,Bax、Cleaved-Caspase-3、Cleaved-Caspase-9蛋白均升高。对照组细胞成团约2~3个,夏枯草组聚集成团减少。与模型组比较,夏枯草组皮下瘤体积,皮下瘤Ki-67及增殖指数均降低。均呈夏枯草剂量依赖性趋势。结论:夏枯草通过调控Bax mRNA及蛋白、Bcl-2 mRNA及蛋白、Caspase-3 mRNA、Caspase-9 mRNA、Cleaved-Caspase-3、Cleaved-Caspase-9表达抑制胶质瘤U87细胞增殖,促进凋亡。

Objective:To investigate the effect and mechanism of Prunella vulgaris on the proliferation and apoptosis of glioma U87 cells.Methods:The cells were divided into the control group,the Prunella vulgaris low,middle and high-dose groups.The cell proliferation,cytotoxicity,cell growth,cell apoptosis,the Bax,Bcl-2 mRNA and protein expression in cell,the Caspase-3,Caspase-9 mRNA,Cleaved-Caspase-3,Cleaved-Caspase-9 protein expression in cell were recorded and compared within groups.The U87 cell tumor-bearing mice were prepared and divided into the model control group,the Prunella vulgaris low,medium and high-dose groups.All groups had been administered for 30 days.The mouse subcutaneous tumor volume,Ki-67 expression and Ki-67 proliferation index in the tumor were collected and compared within groups.Results:Compared with the control group,the number of cell colonies,cell activity,Bcl-2 mRNA and protein expression in the Prunella vulgaris groups decreased,the number of apoptotic cells,Bax,Caspase-3,Caspase-9 mRNA expression,Bax,Cleaved-Caspase-3,the expression of Cleaved-Caspase-9 protein in the Prunella vulgaris groups increased.In the control group,about 2~3 glioma U87 cells clustered,about 0~1 cells in the Prunella vulgaris low-dose group,and no cell clusters were seen in the middle and high-dose Prunella vulgaris groups.Compared with the model group,the volume of subcutaneous tumors,Ki-67 expression and proliferation index of subcutaneous tumors in the Prunella vulgaris groups decreased.All showed a dose-dependent trend of Prunella vulgaris.Conclusion:Prunella vulgaris inhibits the proliferation of glioma U87 cells and promotes apoptosis by regulating the expression of Bax mRNA and protein,Bcl-2 mRNA and protein,Caspase-3 mRNA,Caspase-9 mRNA,Cleaved-Caspase-3,and Cleaved-Caspase-9,showing a dose-dependent trend.