硅纳米颗粒诱导小鼠间充质干细胞成骨分化和迁移的体外研究

In vitro study of osteogenic differentiation and migration of mouse mesenchymal stem cells induced by silica-based nanoparticles

目的通过溶胶-凝胶法合成纳米尺径的硅基生物活性颗粒(SBPs),检测其对间充质干细胞(MSCs)成骨分化和迁移的作用。方法通过溶胶-凝胶法合成SBPs,通过扫描电镜和ZATA电位分析仪检测颗粒的物理特征。在培养基中添加SBPs配置成系列浓度的条件培养基,检测不同浓度的SBPs对小鼠MSCs增殖、成骨分化和迁移的影响。结果SBPs的平均直径为134.3 nm,并且呈均匀分散球形颗粒。不同浓度的SBPs对MSCs增殖无影响;但4μg/mL的SBPs对细胞成骨分化有明显的促进作用;高浓度(>16μg/mL)的SBPs抑制MSCs的成骨分化;4μg/mL的SBPs对MSCs有明显的促进迁移的作用。结论在适宜浓度范围内(1~4μg/mL),SBPs具有促成骨作用和诱导MSCs迁移,未来可能被用来与其他生物材料复合制备新型的骨移植材料。

Objective To assess the effect of the nanosized silicon-based bioactive particles(SBPs)synthesized by sol-gel method on osteogenic differentiation and migration of mesenchymal stem cells(MSCs).Methods SBPs was synthesized by solgel and the physical characteristics of the particles were detected by scanning electron microscopy and ZATA potential analyzer,and SBPs was added to the medium and configured into a series of concentrations of conditioned medium to test the effects of SBPs at different concentrations on the proliferation osteogenic differentiation and migration of MSCs.Results SBPs are spherical particles of uniform size and dispersion with mean diameter of 134.3 nm,and different concentrations of SBPs had no effect on the proliferation of MSCs,but 4μg/mL of SBPs had a significant positive regulatory effect on cell osteogenic differentiation and migration;high concentrations(>16μg/mL)of SBPs inhibited the osteogenic differentiation of MSCs.Conclusion The appropriate concentration(1-4μg/mL)of SBPs can promote the osteogenic differentiation and migration of MSCs,and it can be compounded with other tissue engineering bone materials to synthesize new bone graft materials.