固载离子液体修饰Fe3O4纳米酶用于H2O2和葡萄糖的检测

Immobilized Ionic Liquid Modified FeO Nanoenzyme for the Detection of Hydrogen Peroxide and Glucose in Foods

研究SiO2固载离子液体修饰的磁性纳米粒子(Fe3O4@SiO2@IL)制备,及其H2O2酶活性测试。结果表明,Fe3O4@SiO2@IL具有比辣根过氧化物酶更高的催化活性,并能催化H2O2与过氧化物酶底物3,3’,5,5’-四甲基联苯胺形成蓝色产物。因此,开发H2O2比色检测方法,线性范围为4~100μmol/L,检出限为0.698μmol/L。该方法还可用于葡萄糖的高灵敏度和选择性检测,线性范围为8~200μmol/L,检出限为2.39μmol/L。将该方法用于检测实际样品(如牛奶)中的H2O2,加标回收率为94.0%~107.8%,相对标准偏差为2.24%~3.12%,准确度和精密度较高。具有过氧化物酶活性的Fe3O4@SiO2@IL纳米材料在临床诊断、制药、食品研究等领域具有潜在的应用价值。

Silica-immobilized ionic liquid modified magnetic nanoparticles(Fe3 O4@SiO2@IL) were prepared and applied as a peroxidase mimic for the detection of H2O2. The results showed that Fe3 O4@SiO2@IL had higher catalytic activity than horseradish peroxidase(HRP), and could catalyze the coupling of H2O2 with the peroxidase substrate 3,3’,5,5’-tetramethyl aniline(TMB) to form a blue product. On the basis of this phenomenon, a colorimetric method for H2O2 detection was developed, with a linear range of 4–100 μmol/L and a detection limit of 0.698 μmol/L. The method was also available to sensitively and selectively detect glucose, with a linear range of 8–200 μmol/L and a detection limit of 2.39 μmol/L. The recoveries for this method when applied to detect H2O2 in milk samples ranged from 94.0% to 107.8%, with relative standard deviation(RSD) values of 2.24%–3.12%, showing high accuracy and precision. Therefore, Fe3 O4@SiO2@IL nanomaterials with peroxidase mimic activity can be a promising candidate for application in clinical diagnosis, pharmaceutical, food and other fields.