纳米多孔金电化学传感器构建及其对饮品中抗坏血酸的检测

Preparation of Nanoporous Gold-based Electrochemical Sensor for the Detection of Ascorbic Acid in Drinks

基于纳米多孔金(nanoporous gold,NPG)对抗坏血酸的强电催化氧化作用,通过酸腐蚀制备NPG,构建纳米多孔电极(NPG/GCE)检测饮料中的抗坏血酸。通过循环伏安法和差分脉冲伏安(differential pulse voltammetry,DPV)法对抗坏血酸检测条件进行优化,确定最佳检测条件为pH 4.0的柠檬酸缓冲液。在优化条件下,利用DPV法对抗坏血酸进行检测,结果表明:在6.652 8~160μg/mL的范围内,峰电流密度与抗坏血酸质量浓度呈良好的线性关系;该电极制备简单、灵敏度高、稳定性和抗干扰能力强,对饮料中抗坏血酸的快速检测有良好的应用潜力。

Nanoporous gold(NPG), with high electrocatalytic activity for the oxidation of ascorbic acid was prepared by acid corrosion and used to construct a NPG-modified glassy carbon electrode(GCE) as an electrochemical sensor to detect ascorbic acid in drinks. Cyclic voltammetry(CV) and differential pulse voltammetry(DPV) were used to determine that citrate buffer at pH 4.0 was the best buffer for activating the electrocatalytic activity of NPG. Under optimized conditions, ascorbic acid was detected by DPV. The results showed a good linear relationship between the concentration of ascorbic acid in the range of 6.652 8 to 160 μg/mL and the peak current density. The electrode proved to be easy to prepare and have high sensitivity, good stability and strong interference resistance, indicating great potential for rapid detection of ascorbic acids in drinks.