荧光微球免疫层析方法定量检测水产品中4种硝基呋喃代谢物

Microsphere-based Fluorescence Immunochromatographic Assay for Quantitative Detection of Four Nitrofuran Metabolites in Aquatic Products

采用荧光微球标记抗体作为信号探针,建立荧光微球免疫层析检测方法(fluorescent microsphere immunochromatographic assays,FMICA),并应用于水产品中4种硝基呋喃类兽药代谢物残留量的快速定量检测。结果表明:呋喃妥因、呋喃它酮、呋喃唑酮、呋喃西林代谢物FMICA试纸条的检出限分别为0.004(以1-氨基-乙内酰脲衍生物计算)、0.01(以5-吗啉-3-氨基-2-恶唑烷酮衍生物计算)、0.008(以3-氨基-2-恶唑烷酮衍生物计算)、0.009(以氨基脲衍生物计算)μg/L;检测线性范围分别为0.005~5、0.01~10、0.01~10、0.01~10μg/L;最佳检测时间为10 min,方法特异性良好。水产样品经衍生化处理后,经检测硝基呋喃代谢物的加标回收率在75.88%~104.81%之间,变异系数均小于15%。FMICA方法操作简单、快速、成本低,适用于水产品中硝基呋喃类兽药代谢物快速定量检测。

An immunochromatographic assay(FMICA) using fluorescent microsphere-labeled antibody as the signal probe was developed for rapid quantitative detection of nitrofuran metabolite residues in aquatic products. The limits of detection(LOD) for NPAHD, NPAMOZ, NPAOZ, and NPSEM were 0.004, 0.01, 0.008 and 0.009 μg/L, and the linear ranges were 0.005–5, 0.01–10, 0.01–10, and 0.01–10 μg/L, respectively. The detection process took 10 min. The assay had good specificity. The spiked samples were detected by FMICA after derivatization treatment, and the recoveries of the four analytes ranged from 75.88% to 104.81%, with variation coefficients of less than 15%. Therefore, FMICA proved to be simple, fast, and low-cost and could be suitable for rapid quantitative detection of nitrofuran metabolites in aquatic products.