菲对融合菌株F14胞外聚合物特征的影响

The Characteristics Effect of Phenanthrene on of the Fusion Strain F14 EPS

以菲为研究对象,研究不同浓度的菲诱导对融合菌株F14产生EPS的影响,包括EPS组分含量的变化、对降解效率与荧光性能的影响,并通过FTIR和SEM进一步分析了EPS表面官能团的变化和微观形貌的变化。研究表明:随着菲诱导浓度的增加,EPS组分含量、蛋白峰强度均呈先增后减趋势;随着时间的变化,EPS降解效率呈先增后减趋势。对FTIR分析结果表明:EPS表面的羟基、氨基、酰胺基团和C-O-C基团都参与了降解过程;SEM结果显示:随着菲浓度的增加,EPS表面孔隙逐渐变小,整体变得光滑密实。

Taking phenanthrene as the research object,the effects of different concentrations of phenanthrene on the EPS produced by the fusion strain F14 were studied,including the change of EPS component content,the effect on degradation efficiency and fluorescence performance,furthermore,the changes of EPS surface functional groups and microscopic morphology were further analyzed by FTIR and SEM.Studies have shown that with the increase of the induced concentration of phenanthrene,the content of EPS components and the intensity of protein peaks both increase first and then decrease;As time changes,the EPS degradation efficiency shows a trend of first increasing and then decreasing.The FTIR analysis results show that the hydroxyl,amino,amide and C-O-C groups on the surface of EPS are all involved in the degradation process.SEM results show that with the increase of phenanthrene concentration,the pores on the EPS surface gradually become smaller,and the whole becomes smooth and dense.