基于迁移芯片木蝴蝶总黄酮类对肝癌细胞迁移的影响研究

Effect of total flavonoids of oroxyli semen on the migration of liver cancer cells based on microfluidic chip technology

目的研究木蝴蝶总黄酮体外抑制肝肿瘤细胞迁移作用及其可能的作用机制。方法应用微流控芯片技术开展木蝴蝶总黄酮对肝癌细胞迁移的影响研究,采用RT-PCR、Western blot法检测木蝴蝶总黄酮对肝癌细胞相关基因和蛋白质表达的影响。结果微流控芯片结果显示,木蝴蝶总黄酮对肝癌细胞24 h相对迁移率为16.59%,48 h相对迁移率为8.06%;RT-PCR和Western blot结果表明,木蝴蝶总黄酮能降低p38 mRNA和蛋白质的表达(P<0.05),能降低p-p38蛋白质的表达(P<0.01)。结论木蝴蝶总黄酮在体外具有显著抑制肿瘤细胞迁移的作用,其作用机制可能与减少p-p38蛋白质的表达,即减少p38蛋白的活化有关。

Objective To determine the inhibitory effect of total flavonoids of oroxyli semen(TF)on the migration of liver cancer cells and its possible mechanism.Methods The effect of TF on the liver cancer cell migration was determined by microfluidic chip technology.RT-PCR and Western blot were used to detect the effect of TF on the expression of related genes and proteins.Results The microfluidic chip showed that the relative migration rate of TF to liver cancer cells at 24 h was 16.59%,and at 48 h was 8.06%.RT-PCR and Western blot showed that TF down-regulated the expression of p38 mRNA and protein(P<0.05),and down-regulated the expression of p-p38 protein(P<0.01).Conclusion TF has a significant inhibitory effect on the tumor cell migration in vitro,and the mechanism may be related to the down-regulation of the expression of p-p38 protein,namely reducing the activation of p38 protein.