表油菜素内酯对夏黑葡萄幼苗生长的影响

Study on epibrassinolide(EBR)on growth and physiological response of Summer Black grape seedlings

为研究叶面喷施表油菜素内酯(EBR)对葡萄幼苗生长的影响,采用盆栽试验,以夏黑葡萄幼苗为材料,叶面喷施不同浓度(0、0.5、1.0、1.5、2.0 mg·L^(-1))的EBR,测定EBR对葡萄幼苗生物量、光合特性、抗氧化系统的影响。结果表明,叶面喷施不同浓度的EBR提高了葡萄幼苗的生物量、根冠比和光合色素含量,降低了叶绿素a/b;除了叶温下蒸气压亏缺值有所降低,幼苗叶片的光合参数(净光合速率、气孔导度、蒸腾速率和胞间CO_(2)浓度)升高;叶片的抗氧化酶活性、游离脯氨酸和可溶性蛋白质含量均升高,且均在EBR浓度为1.0 mg·L^(-1)和1.5 mg·L^(-1)时升高幅度较大;喷施1.0 mg·L^(-1)和1.5 mg·L^(-1)EBR时,叶片的相对电导率降低;喷施1.5 mg·L^(-1)EBR时,幼苗根、茎的可溶性糖含量升高;喷施0.5 mg·L^(-1)和1.0 mg·L^(-1)EBR时,幼苗叶片的可溶性糖含量升高。总体上,叶面喷施1.0 mg·L^(-1)EBR能够促进夏黑葡萄幼苗的生长,并且能够提高幼苗的抗氧化性。

In order to study the effect of foliar application of epibrassinolide(EBR)on growth of grape seedlings.A pot experiment was conducted to determine the effect of EBR on biomass,photosynthetic characteristics and antioxidant system of Summer Black grape seedlings by foliar spraying with different concentrations of EBR(0,0.5,1.0,1.5,2.0 mg·L^(-1)).The results showed that foliar application of different concentrations of EBR increased the biomass and root shoot ratio of grape seedlings,and photosynthetic pigment content showed the same trend,but the chlorophyll a/b decreased;In addition to the decrease of vapor pressure deficit at leaf temperature,EBR increased photosynthetic parameters(photosynthetic rate,conductance stomatal,transpiration rate,intercellular CO_(2) concentration)of seedling leaves;Activities of antioxidant enzymes,contents of free proline and soluble protein in leaves of Summer Blackgrape seedlings were increased by different concentrations of EBR,the better performance was 1.0 mg·L^(-1)and 1.5 mg·L^(-1).When EBR concentration reached 1.0 mg·L^(-1)and 1.5 mg·L^(-1),relative conductivity of the leaves was reduced;When EBR concentration was 1.5 mg·L^(-1),soluble sugar content of roots and stems of seedlings was improved;When EBR concentration reached 0.5 mg·L^(-1)and 1.0 mg·L^(-1),soluble sugar content of seedling leaves was increased.In general,foliar spraying of 1.0 mg·L^(-1)EBR could promote growth of Summer Black grape seedlings and increase the antioxidant capacity of the seedlings.