SBPase基因超表达对烟草生长的影响

Effects of SBPase gene overexpression on tobacco growth

【目的】探究SBPase基因超表达对烟草农艺性状与光合特性的影响。【方法】采用含SBPase基因的表达载体转化烟草叶片,获得转基因烟草株系L1、L2、L3,以野生型烟草WT为对照,利用微滴式数字PCR技术筛选低拷贝转化植株,并测定转基因烟草农艺性状与光合特性。【结果】3个转基因烟草株系植株的茎围、叶长均显著高于野生型烟草(P<0.05),茎围分别增加了52.47%、21.34%和49.17%,叶长分别增加了30.77%、25.96%和39.10%;叶宽均极显著高于野生型烟草(P<0.01),分别增加了55.48%、53.82%、65.39%;叶片数和株高均高于野生型烟草,但未达到显著差异水平(P>0.05)。3个转基因烟草株系的净光合速率显著高于野生型烟草(P<0.05),分别提高了88.2%、43.6%和27.1%;蒸腾速率、气孔导度均略高于野生型烟草,但未达到显著差异水平(P>0.05);胞间CO_(2)浓度与野生型烟草达到显著差异水平(P<0.05),分别提高了13.0%、4.4%和4.3%。【结论】SBPase基因超表达使烟草光合性能大幅度提高,能够积累更多的光合作用产物,从而促进自身生长。

【Objective】To investigate the effects of SBPase gene overexpression on agronomic traits and photosynthetic characteristics of tobacco.【Methods】Tobacco leaves were transformed with expression vectors containing SBPase gene to obtain transgenic tobacco lines L1,L2,and L3.Wild-type tobacco WT was used as control,and low-copy transgenic plants were screened by microdrop digital PCR,and agronomic traits and photosynthetic characteristics of transgenic tobacco were measured.【Results】It was found that the stem circumference and leaf length of three transgenic tobacco lines was significantly(P<0.05)higher than that of wild-type,the stem circumference increased by 52.47%,21.34%,and 49.17%,respectively;leaf length increased by 30.77%,25.96%,and 39.10%,respectively.The leaf width was extremely significantly higher(P<0.01)than that of wild-type tobacco by 55.48%,53.82%,and 65.39%,respectively.The number of leaves and plant height of transgenic tobacco were higher than that of wild-type tobacco,but the difference were not significant(P>0.05).The net photosynthetic rates of three transgenic tobacco lines were significantly(P<0.05)higher than those of wild-type tobacco by 88.2%,43.6%,and 27.1%,respectively.The transpiration rate and stomatal conductance were slightly higher than those of wild-type tobacco,but the differences were not significant(P>0.05).Intercellular CO_(2) concentration was significantly(P<0.05)higher than that of wild-type tobacco,which was 13.0%,4.4%,and 4.3%higher,respectively.【Conclusion】The overexpression of SBPase gene greatly improved the photosynthetic performance of tobacco,which could accumulate more photosynthetic products and promote its own growth.