丙泊酚对小胶质细胞焦亡的影响及其潜在机制

Effect and potential mechanism of propofol on microglial pyroptosis

目的探讨丙泊酚对小胶质细胞焦亡的影响及其潜在机制。方法250μmol/L丙泊酚处理小鼠小胶质BV2细胞3 h,检测线粒体膜电位、线粒体活性氧、线粒体呼吸链复合物Ⅰ酶活性及NLRP3、ASC、caspase-1、GSDMD、IL 1β、LC3B、p62、COX4I1、cathepsin B表达。结果丙泊酚引起BV2细胞线粒体损伤、NLRP3炎症小体介导的焦亡,并抑制线粒体自噬。结论丙泊酚可通过抑制线粒体功能介导BV2细胞焦亡。

Objective To study the effect of and potential mechanism of propofol on microglial pyroptosis.Methods After treated with 250μmol/L propofol for 3 h,mitochondrial membrane potential,reactive oxygen species,respiratory chain complex I enzyme activity,and expression of NLRP3,ASC,caspase-1,GSDMD,IL-1β,LC3B,p62,COX4I1,and cathepsin B were determined in mouse microglia BV2.Results Propofol induced mitochondrial damage and NLRP3 inflammasome mediated pyroptosis,and suppressed mitophagy in BV2 cells.Conclusion Propofol can mediate pyroptosis of BV2 cells via inhibiting mitochondrial function.