黄连素联合他莫昔芬对耐药乳腺癌细胞T-47D生物学行为的影响

Effect of berberine and tamoxifen on biological behavior of drug-resistant breast cancer T-47D cells

目的观察黄连素(BBR)联合他莫昔芬(TAM)对耐药性乳腺癌细胞生物学行为的影响。方法乳腺癌耐药细胞株T-47D分为对照组、TAM组、BBR组、TAM+BBR组,采用CCK-8、划痕实验、流式细胞术、Westernblot分别检测细胞增殖、迁移、细胞周期及p-AKT、p-AMPK表达。结果与对照组比较,TAM组、BBR组与TAM+BBR组细胞增殖率明显降低,而细胞迁移抑制率、细胞周期阻滞率及p-AKT/AKT、p-AMPK/AMPK表达均明显增高(P<0.01或0.05),以TAM+BBR组最为显著(P<0.01)。结论BBR联合TAM可能通过激活PI3K/AKT和MAPK通路,抑制T-47D细胞增殖、迁移,阻滞细胞周期在G1期。

Objective To observe the effect of berberine(BBR)and tamoxifen(TAM)on biological behavior of drug resistant breast cancer cells.Methods Drug-resistant breast cancer T-47D cells were divided into control,TAM,BBR,and TAM+BBR groups.Cell proliferation,migration,cell cycle,and expression of p-AKT and p-AMPK were respectively detected by CCK-8,scratch test,flow cytometry,and Western blot.Results Compared with contrl group,cell proliferation decreased,while cell migration inhibition,cell cycle arrest,and expression of p-AKT/AKT and p-AMPK/AMPK increased in TAM,BBR and TAM+BBR groups(P<0.01 or 0.05),especially in TAM+BBR group(P<0.01).Conclusion Combined BBR and TAM might inhibit cell proliferation and migration and block cell cycle in G1 phase via activating PI3K/AKT and AMPK pathways.