表儿茶素对急性肺损伤小鼠抗氧化应激和抗炎作用研究

Study on the Anti-oxidative Stress and Anti-inflammatory Effect of Epicatechin on Acute Lung Injury in Mice

【目的】探讨表儿茶素(EC)对脂多糖(LPS)诱导急性肺损伤(ALI)小鼠氧化应激和炎症的调节作用。【方法】采用气管滴注法建立ALI模型,通过测定肺组织湿干质量比(W/D)评估其水肿程度,检测过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的活性以及丙二醛(MDA)和蛋白质羰基的含量以评估其体内抗氧化损伤的作用,检测小鼠血清、支气管肺泡灌洗液(BALF)和肺组织中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量、肺组织内一氧化氮(NO)的含量以及磷酸化的P38分裂原激活的蛋白激酶(P38)、细胞外信号调节激酶(ERK)和c-jun N端激酶(JNK)的表达水平评估其抗炎症作用。【结果】EC(40 mg/kg)预处理能降低ALI小鼠的肺组织的W/D值和水肿程度,提高CAT、GSH-Px和SOD等酶的活性,降低MDA和蛋白质羰基的含量提升其体内抗氧化活性,降低IL-1β、IL-6、TNF-α和NO的含量并降低肺组织p-JNK/JNK(P<0.05)、p-ERK/ERK(P<0.01)和p-P38/P38(P<0.01)的比值。【结论】EC可以调节氧化应激和炎症反应改善小鼠的ALI。

[Purpose]To investigate the regulation effect of epicatechin(EC)on lipopolysaccharide(LPS)induced acute lung injury(ALI)mice oxidative stress and inflammation.[Methods]A tracheal instillation method was used to establish ALI model in BALB/c mice.The degree of edema of lung tissues were assessed by measuring the wet/dry weight ratio(W/D).Its anti-oxidative damage effects also were detected by evaluating catalase(CAT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)activities,malondialdehyde(MDA)and protein carbonyl content.Serum,bronchoalveolar lavage fluid(BALF)and lung tissues interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and nitric oxide(NO)content and expression of phosphorylated P38 mitogen activated protein kinases(P38),extracellular regulated kinases(ERK)and c-jun N-terminal kinase(JNK)were detected to assess its anti-inflammatory effects.[Results]EC(40 mg/kg)pretreatment effectively decreased the W/D ratio of lung tissue to improve the degree of edema,and increased the activity of CAT,GSH-Px and SOD and reduced the level of MDA and protein carbonyl to improve its antioxidant activity.EC(40 mg/kg)also reduced the content of IL-1β,IL-6,TNF-αand NO and decreased the ratio of p-JNK/JNK(P<0.05),p-ERK/ERK(P<0.01)and p-P38/P38(P<0.01)in lung tissues.[Conclusion]EC can regulate oxidative stress and inflammation and improve ALI in mice.