山药多糖对丙烯酰胺诱导的巨噬细胞氧化损伤的保护作用

Protective effect of Rhizoma dioscoreae polysaccharides on oxidative damage induced by acrylamide in macrophages

该文研究山药多糖对丙烯酰胺(acrylamide,AM)诱导的巨噬细胞氧化损伤的保护作用。以AM诱导小鼠巨噬细胞(RAW264.7)损伤,山药多糖进行保护,检测山药多糖对细胞增殖、吞噬活力以及氧化应激的影响;并构建生物大分子(蛋白、脂类、DNA)氧化损伤模型,评价山药多糖对生物大分子的保护作用。结果表明,与正常对照组相比,不同质量浓度的山药多糖对细胞增殖影响无显著性差异。与诱导组(AM)相比,不同浓度的山药多糖能显著促进细胞生长和提高细胞吞噬活力。山药多糖预处理能有效抑制细胞活性氧产生,减少丙二醛累积,提高细胞超氧化物歧化酶活性。此外,山药多糖对生物大分子也具有良好的保护作用。山药多糖可以通过提高细胞抗氧化能力,抑制细胞的氧化应激,抑制生物大分子氧化损伤,从而有效保护丙烯酰胺诱导的巨噬细胞氧化损伤。该研究为控制丙烯酰胺毒性及山药多糖的开发利用提供理论依据。

The aim of this study was to investigate the protective effect of Rhizoma dioscoreae polysaccharides on acrylamide(AM)-induced oxidative damage in mouse macrophages.AM-induced macrophage(RAW264.7)injury was first established,and Rhizoma dioscoreae polysaccharides were used to protect it.The effects of Rhizoma dioscoreae polysaccharides on cell proliferation,phagocytic activity and oxidative stress were detected.Additionally,the oxidative models were applied to evaluate the protective effect of yam polysaccharides on biological macromolecules(protein,lipid,DNA).The results showed that different concentrations of polysaccharides had no significant effect on cell proliferation,compared with the normal control group.Compared with the induction(AM)group,Rhizoma dioscoreae polysaccharides could significantly promote cell growth and improve phagocytic activity.Polysaccharide pretreatment could effectively inhibit the production of ROS,reduce the accumulation of MDA and enhance the activity of SOD.In addition,Rhizoma dioscoreae polysaccharides also exerted a good protective effect on biological macromolecules.Rhizoma dioscoreae polysaccharides could effectively protect macrophages from AM-induced oxidative damage by improving cell antioxidant capacity,inhibiting cell oxidative stress and macro-molecule oxidation.This study provides a theoretical basis for controlling the toxicity of acrylamide and for the potential development and utilization of Rhizoma dioscoreae polysaccharides.