罗非鱼鳞胶原肽螯合锌抗氧化及抑菌活性研究

Antioxidant and antibacterial activities of collagen peptide chelated zinc from tilapia scales

以罗非鱼鳞为原料,制备了胶原肽及胶原肽螯合锌,研究并比较了其体外抗氧化及抑菌活性。基质辅助激光解析电离飞行时间质谱图表明了罗非鱼鳞胶原肽螯合锌的生成。氨基酸分析显示,罗非鱼鳞胶原肽富含羟脯氨酸、甘氨酸以及天冬氨酸,与锌离子螯合后,氨基酸的组成与比例有所变化,其中天冬氨酸与谷氨酸占总氨基酸的比例分别由19.72%和8.79%增加到22.73%和9.71%。体外抗氧化实验表明,罗非鱼鳞胶原肽螯合锌对DPPH自由基、羟自由基的清除率IC_(50)值分别为3.69和6.59 g/L,并且具有较强的Fe^(3+)还原能力,螯合锌的抗氧化活性明显优于胶原肽。抑菌实验显示罗非鱼鳞胶原肽螯合锌对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和白色假丝酵母等4种指示菌均表现出明显的抑制作用,而罗非鱼鳞胶原肽无抑菌效果。由此可见,罗非鱼鳞胶原肽螯合锌有望作为一种潜在的抗氧化、抑菌剂。

Collagen peptide and collagen peptide chelated zinc were prepared from tilapia scales and evaluated in terms of their in vitro antioxidant and antibacterial activities.The matrix assisted laser desorption ionization time-of-flight mass spectra confirmed the generation of collagen peptide chelated zinc.Amino acid analysis indicated that the collagen peptide was rich in hydroxyproline,glycine and aspartic acid.After chelation with zinc ions,the amino acid composition and proportion were significantly changed.Especially,the proportion of aspartic acid and glutamic acid in total amino acids increased from 19.72%and 8.79%to 22.73%and 9.71%,respectively.In vitro antioxidant results showed that the IC_(50) values of collagen peptide chelated zinc were 3.69 and 6.59 g/L for DPPH and·OH free radical scavenging ability,respectively.Furthermore,collagen peptide chelated zinc displayed strong ferric iron reducing power.The antioxidant activity of collagen peptide chelated zinc was superior to that of collagen peptide.Although collagen peptide from tilapia scales showed no antibacterial activity,collagen peptide chelated zinc exhibited significant antibacterial activity against Bacillus subtilis,Escherichia coli,Staphylococcus aureus and Candida albicans.Therefore,collagen peptide chelated zinc from tilapia scales could be used as a potential antioxidant and antibacterial agent.