QuEChERS-UPLC-MS/MS同时测定动物性食品中24种残留兽药的基质效应

Matrix effect in analysis of 24 veterinary drug residues in animal-derived foods by QuEChERS-UPLC-MS/MS

该文通过评价离子抑制率,系统地考察了鲫鱼、鸡肉、鸭胗、猪肝、牛肉、猪肉中24种兽药的基质效应及同位素内标、基质匹配标准曲线对基质效应的补偿效果。结果表明,磺胺间二甲氧嘧啶、磺胺邻二甲氧嘧啶、环丙沙星、恩诺沙星、诺氟沙星、红霉素、金刚烷胺需采用同位素内标校正基质效应,使用内标后,7种兽药的基质效应在6种基质中降低了0.15%~66.94%(n=3);其余17种兽药需采用基质匹配标准曲线校正,经空白猪肉基质匹配标准曲线校正后17种兽药的加标回收率提高了11.71%~59.47%(n=3),基质效应对定量结果的干扰得到了有效补偿。该研究为动物性食品中多兽残快速准确检测提供了一定的参考依据。

The accuracy and sensitivity of the result can be influenced by matrix effects.By evaluating ion suppression rate,the matrix effects of 24 veterinary drugs in carp,chicken,duck gizzard,pork liver,beef and pork were systematically evaluated in this study.The compensation effect on the matrix effect by using isotope internal standard and the matrix-matched standard curve were also been evaluated.The results showed that sulfamethoxine,sulfadoxine,ciprofloxacin,enrofloxacin,norfloxacin,erythromycin and amantadine were appropriate for the isotope internal standard calibration method.When adopting this method,the matrix effects of these 7 veterinary drugs decreased by 0.15%-66.94%(n=3)in 6 kinds of the matrix.The other 17 kinds of veterinary drugs were appropriate for adapting the matrix-matched standard curve method to calibrate matrix effect since the recoveries of the 17 kinds of veterinary drugs increased by 11.71%-59.47%(n=3)after being calibrated by blank pork matrix-matched standard curve.The adverse interference of quantification results caused by the matrix effect was successfully compensated by both methods.This study provides a reference for the rapid and accurate detection of veterinary drug residues in animal-derived food.