苗药滚山珠HPLC指纹图谱及化学模式识别研究

Study on HPLC fingerprint and chemical pattern recognition of Miao medicine Prionobelum qianensis Chen

利用HPLC指纹图谱结合化学模式识别技术对滚山珠药材进行质量评价。采用Agilent ZORBAX SB-C 18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%乙酸水溶液为流动相梯度洗脱,检测波长254 nm,流速0.8 mL/min,进样量2μL,柱温25℃。然后利用相似度评价、聚类分析、主成分分析和正交偏最小二乘别分析等化学模式识别方法,对不同来源的滚山珠药材质量进行分析和评价。所建立的HPLC指纹图谱共识别、标定了22个共有峰,经鉴定其中的1个共有峰为酪氨酸,24批不同来源滚山珠的相似度在0.739~0.957。经正交偏最小二乘判别分析筛选出了导致不同批次药材质量差异的5个共有峰。本研究建立的滚山珠HPLC指纹图谱结合化学模式识别的方法简单、可靠,方法重复性好、专属性强,可为滚山珠的质量控制提供了依据和参考。

The HPLC fingerprints and chemical pattern recognition were studied to evaluate the quality of Prionobelum qianensis Chen.The chromatographic separation was performed on a Agilent ZORBAX SB-C 18 column(250 mm×4.6 mm,5μm).Methanol-0.1%acetic acid solution was used as mobile phase with gradient elution.The detection wavelength was set at 254 nm,the flow rate was 0.8 mL/min,the injection volume was 2μL,and the column temperature was 25℃.The chemical pattern recognition of similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares analysis were used to analyze and evaluate the quality of P.qianensis.A total of 22 common peaks were identified and demarcated by HPLC fingerprint,one of them was identified as tyrosine.The results about the chemical pattern recognition showed that the similarities of those fingerprints were between 0.739 and 0.957.Furthermore,the quality difference of those P.qianensis was mainly caused by 5 common peaks according to the results of orthogonal partial least squares discriminant analysis.In conclusion,the HPLC fingerprint combined with chemical pattern recognition provide of P.qianensis established in this study is simple,reliable,reproducible,and specific,which provides a basis and reference for the quality control of P.qianensis.