罗非鱼肠道真菌Alternaria tenuissima SCSIO41701中的抗菌化合物

Antibacterial compounds from the tilapia intestine derived fungus Alternaria tenuissma SCSIO41701

对罗非鱼肠道来源真菌Alternaria tenuissima SCSIO41701大米培养基发酵产物中抑制罗非鱼无乳链球菌和海豚链球菌生长的活性化合物进行研究。结合抗菌活性追踪,采用硅胶柱层析和HPLC等色谱技术对其抗菌活性组分进行分离纯化,并通过1H NMR和13 C NMR波谱技术,以及与文献报道的NMR数据相比较鉴定化合物结构。共分离鉴定了9个化合物,分别是aternariol(1)、aternariol methyl ether(2)、3-hydroxyalternariol 5-O-methylether(3)、(-)-altenuene(4)、(+)-isoaltenuene(5)、altenusin derivative(6)、alternariphent(7)、talaroflavonene(8)、alternarienonic acid B(9)。其中含量最高的化合物1抗菌活性最强,在25μg/disc的浓度下对罗非鱼病原菌无乳链球菌和海豚链球菌均有显著抑制作用,抑菌圈直径均为10 mm,且其MIC值均为12.5μg/mL。这是首次报道化合物1抗罗非鱼链球病病原菌活性。

This study investigated antibacterial compounds from the rice medium of the tilapia intestinal derived fungus Alternaria tenuissima SCSIO41701.The compounds were separated by silica gel column chromatography,HPLC and other chromatographic techniques.The structures of the compounds were identified by 1H NMR,13 C NMR,and comparison with the NMR data of literatures.Totally,nine compounds were isolated from the antibacterial fractions of the tilapia intestines derived fungus Alternaria tenuissma.These compounds were identified as aternariol(1),aternariol methyl ether(2),3-hydroxyalternariol 5-O-methylether(3),(-)-altenuene(4),(+)-isoaltenuene(5),altenusin derivative(6),alternariphent(7),talaroflavonene(8),and alternarienonic acid B(9).Only compound 1 showed obvious inhibition activity against the growths of Streptococcus iniae and S.agalactiae at 25μg/dics with inhibition zones of 10 mm,and its MIC values were 12.5μg/mL.