基于斑马鱼模型的竹柳多糖对感觉毛细胞保护活性的研究

Study on the protective activity of polysaccharides from bamboo willow on sensory hair cells using zebrafish model

选用斑马鱼为实验动物,通过表型观察、荧光标记、凋亡检测等技术手段,研究竹柳来源多糖对机体的生物学影响以及其对卡那霉素诱导的感觉毛细胞损伤的保护活性与作用机制。研究结果显示,竹柳多糖的作用浓度为30、40、50μg/mL时,其单独或与卡那霉素共同作用斑马鱼后,均无明显的毒性或致畸作用。但是在此浓度下,该多糖可有效减少感觉毛细胞内ROS的积聚,并抑制感觉毛细胞发生凋亡,最终对卡那霉素诱导的感觉毛细胞损伤起到保护功效。该研究为竹柳多糖在药源性耳聋疾病防治药物开发领域的应用提供了依据。

In this study,we selected zebrafish as experimental animals to study the biological effects on the organism and the protective activity and mechanism of polysaccharides from bamboo willow against kanamycin induced sensory hair cell injury by phenotype observation,fluorescence labeling and apoptosis detection.The results showed that when the concentration of polysaccharide was 30,40,50μg/mL,it had no obvious toxicity or teratogenic effects after acting on zebrafish alone or with kanamycin.However,at the same concentration the polysaccharides could effectively reduce the accumulation of ROS in sensory hair cells,and inhibit the apoptosis of sensory hair cells.As a result,polysaccharides from bamboo willow played a protective effect on kanamycin-induced sensory hair cell damage.This study provided a basis for the application of polysaccharides in the development of drugs for the prevention and treatment of drug-induced deafness.