AMOTL2与FKBP51表达水平与胶质母细胞瘤病人预后的关系

Relationship between AMOTL2 and FKBP51 expression and prognoses of glioblastoma patients

目的探讨胶质母细胞瘤(GBM)类血管动蛋白-2(AMOTL2)与FK506结合蛋白51(FKBP51)表达变化及临床意义。方法收集2016年6月~2019年6月手术切除的GBM标本92例,以取同期颅脑损伤内减压术切除的非肿瘤脑组织48例为对照。采用免疫组化染色法检测AMOTL2、FKBP51表达。GBM病人术后随访2年,记录无进展生存期和总生存期。结果GBM组织AMOTL2低表达率、FKBP51高表达率均明显高于对照组(P<0.05)。GBM组织AMOTL2表达水平与P53、EGFR呈明显负相关(P<0.05),FKBP51表达水平与P53、EGFR呈明显正相关(P<0.05)。Cox比例回归风险模型分析显示,AMOTL2低表达、FKBP51高表达是GBM生存预后不良的独立危险因素(P<0.05)。生存曲线分析显示,AMOTL2高表达组病人无进展生存期、总生存期较低表达组均明显延长(P<0.05),FKBP51高表达组无进展生存期、总生存期较低表达组均明显缩短(P<0.05)。结论GBM组织AMOTL2呈低表达,而FKBP51呈高表达,二者均与P53、EGFR表达相关,与病人预后密切相关。

Objective To explore the expression changes of angiomotin-2(AMOTL2)and FK506 binding protein 51(FKBP51)in glioblastoma(GBM)tissues and their clinical significance.Methods The expression levels of AMOTL2 and FKBP51 were detected in GBM tissues obtained from 92 GBM patients who underwent microsurgery from June 2016 to June 2019 and in non-tumor cerebral tissues obtained from 48 patients(control)with traumatic brain injury who underwent decompression during the same period by immunohistochemical staining.The patients with GBM were followed up for 2 years,and the progression-free survival(PFS)and overall survival(OS)were recorded.Results The low expression rate of AMOTL2 and the high expression rate of FKBP51 in GBM tissues were significantly higher than those in the control group(P<0.05).The expression level of AMOTL2 in GBM tissues was significantly negatively correlated with P53 and EGFR(P<0.05),and the expression level of FKBP51 was significantly positively correlated with P53 and EGFR(P<0.05).Cox proportional regression risk model analysis showed that low expression of AMOTL2 and high expression of FKBP51 were independent risk factors for poor survival prognoses of GBM patients(P<0.05).Survival curve analysis showed that the PFS and OS of patients in the AMOTL2 high-expression group were significantly prolonged compared to the low-expression group(P<0.05).The PFS and OS of patients in the FKBP51 high-expression group were significantly shortened compared to the low-expression group(P<0.05).Conclusions The expression of AMOTL2 in GBM tissue is low,while the expression of FKBP51 is high.Both of them are related to the expression of P53 and EGFR in GBM tissues,and are closely related to the GBM patient's prognosis.