摘要
为探究添加外源氨基酸对侧孢短芽孢杆菌S62-9(Brevibacillus laterosporus S62-9,BL)分泌抗菌肽Brevilaterin的影响,以BL发酵液的抗菌活性和Brevilaterin的组分变化为指标,分别采用琼脂扩散法和高效液相色谱法进行了分析。结果表明:添加L-缬氨酸、L-甲硫氨酸和2-氧代-3-甲基丁酸均能提高发酵液抗菌活性,抑菌直径最高可增加18.08%。添加外源氨基酸还能改变Brevilaterin的组分构成。添加L-甲硫氨酸后发酵液中只有抗菌肽组分Brevilaterin B和C,且二者间的相对比例发生反转;而添加L-缬氨酸或2-氧代-3-甲基丁酸还能促进BL分泌多种新组分。添加外源氨基酸能有效提高发酵液抗菌活性并改变抗菌肽的组分构成,研究结果旨在为人工调控微生物合成新型、高效的抗菌肽奠定一定的理论基础。
To explore the effect of addition of exogenous amino acids on Brevibacillus laterosporus S62-9(BL)secreted Brevilaterin,the antibacterial activity of BL fermentation broth and the composition change of Brevilaterin were analyzed by agar diffusion method and high performance liquid chromatography,respectively.The results showed that the addition of L-valine,L-methionine and 2-oxo-3-methyl-butyric acid could increase the antibacterial activity of the fermentation broth,and the maximum antibacterial diameter could increase by 18.08%.Furthermore,the addition of exogenous amino acids could also change the components of Brevilaterin.The addition of L-methionine could induce BL only to secrete Brevilaterin B and C,and the relative ratio between the two was reversed.The addition of L-valine or 2-oxygen-3-methyl-butyric acid also promoted BL to secrete a variety of new components.In conclusion,adding exogenous amino acids could effectively improve the antibacterial activity of the fermentation broth and even change the components of antimicrobial peptides,which might provide a theoretical foundation for the artificial regulation of microbial synthesis of novel and efficient antimicrobial peptides.
作者
王星星
陈洲
韩盼盼
刘杨柳
李思霆
贾英民
WANG Xingxing;CHEN Zhou;HAN Panpan;LIU Yangliu;LI Siting;JIA Yingmin(School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)
出处
《食品科学技术学报》
CAS
CSCD
北大核心
2021年第5期74-81,共8页
Journal of Food Science and Technology
基金
国家自然科学基金资助项目(31801510,31771951)
北京市自然科学基金-市教委联合重点项目(KZ201810011016)。
关键词
侧孢短芽孢杆菌
抗菌肽
外源氨基酸
抗菌活性
组分构成
Brevibacillus laterosporus
antimicrobial peptide
exogenous amino acid
antibacterial activity
component constitution