双光子荧光显微系统中波前像差对三维点扩散函数的影响

Effect of wavefront aberration on 3D point-spread function in two-photon fluorescence microscopy

双光子荧光显微系统被广泛应用于神经科学、免疫科学等生物医学研究,但由于生物组织的固有光学属性、样品放置等因素造成波前像差,使得显微成像质量易受影响.本文通过数值仿真分析了各泽尼克多项式所描述的像差及其对三维点扩散函数的影响,并以球差为例进行了实验验证.

Two-photon microscopy has been widely adopted in biomedical study,such as neuroscience and immunology.However,suffering from wavefront aberrations resulting from the inherent optical properties and sample alignment,etc,the imaging quality will be degraded.Here,we analyzed the effect of wavefront aberrations,described by Zernike polyminals,on 3D point-spread function in two-photon fluorescence microscopy.Besides,we performed experiments for verification with the spherical aberration as an example.