表面活性剂胁迫培养对4株细菌生长的影响

Impacts of Surfactant Stress on the Growth of Four Kinds of Bacteria

为研究表面活性剂胁迫对微生物生长的影响,本实验选取4株细菌作为实验材料:枯草芽孢杆菌(BaciLLus subtiLis)、简单芽孢杆菌(BaciLLus simpLex)、大肠杆菌(Escherichia coLi)、变形杆菌(Proteus vuLgaris),用传统微生物培养方法,选择两种表面活性剂聚乙二醇6000(PEG6000)、十二烷基硫酸钠(SDS)在不同浓度下对4株细菌进行胁迫培养,通过测定4株细菌的生长曲线,探讨表面活性剂对细菌生长的影响。结果表明:变形杆菌对PEG6000表现出较高的耐受性,另外3株细菌的生长受到抑制;SDS低浓度(0.2g/L)即对细菌的生长有明显的抑制作用,两种表面活性剂对4株细菌的毒性表现为SDS>PEG6000。

To study the impacts of surfactant stress on microbial growth,this project took four strains of bacteria as the experimental materials including Bacillus subtilis,Bacillus simplex,Escherichia coli and Proteus vulgaris.The traditional methods of microbial culture;selected two kinds of surfactants such as polyethylene glycol 6000(PEG6000)and sodium dodecyl sulfate(SDS)were used to culture four strains of bacteria under varying concentrations to explored the impacts of surfactants on the growth of bacteria through measuring the growth curves of four strains of bacteria.The results showed that Proteus vulgaris showed a particular higher tolerance to PEG6000 than others that were apparently inhibited;the Low concentration of SDS(0.2g/L)significantly inhibited the growth of the bacteria,and the toxicity of the two surfactants to the four strains of bacteria was sequenced as SDS>PEG6000.