纤花香茶菜苗期抗旱性鉴定及抗旱生理特性初步研究

Preliminary Study on Drought-resistant Identification and Physiological Characteristics of Drought Resistance of Isodon lophanthoidesat Seedling Stage

采用盆栽反复干旱法对17份纤花香茶菜种质资源进行苗期抗旱性鉴定,测定其冠层萎蔫比例、叶片含水量、株高等10个指标,应用隶属函数法等方法对其抗旱性进行综合评价,并对有抗旱性差异的种质进行生理特性研究。结果表明,平均隶属函数值与叶长和叶面积的相关性较大;主成分分析将10个指标归纳为光合因子、产量因子和生长因子3个主成分,累计贡献率为74.438%;聚类分析将供试种质分为强抗旱型、中抗旱型、弱抗旱型和抗旱敏感型四种类型;抗旱性不同的种质对干旱胁迫的生理应答策略不同,弱抗旱型种质需要做出更强烈的生理应答来应对干旱胁迫。结合课题组前期芽期抗旱性鉴定结果认为,R 16两生育期均具有优良抗旱特性。

In this paper, the drought resistance of 17 germplasm resources of Isodon lophanthoides at seedling stage was identified by pot with repeated drought method. Ten indexes including canopy wilting ratio, leaf water content and plant height were measured, and the drought resistance of the germplasms was evaluated comprehensively by membership function method. And, the physiological characteristics of germplasms with different drought resistance were also studied. The results showed that the average membership function had great correlation with leaf length and leaf area. The 10 indexes were classified into three principal components, namely photosynthetic factor, yield factor and growth factor, with a cumulative contribution rate of 74.438% based on principal component analysis(PCA). Further, the tested germplasms was divided into four types including strong drought-resistant type, medium drought-resistant type, weak drought-resistant type and drought-sensitive type. Germplasms with different drought resistance had different physiological response strategies to drought stress and germplasms with weak drought resistance needed to make stronger physiological response to drought stress. Combined with the results of drought resistance identification in the early bud stage, the germplasm R 16 had excellent drought resistance characteristics in both growth stages.