甲醇老化处理对黄瓜种子发芽的影响

Effect of Methanol Aging Treatment on Germination of Cucumber (Cucumis sativus L.) Seeds

用50%甲醇溶液浸泡黄瓜种子,进行人工老化处理。测量幼苗的干鲜重、胚轴长、最大根长、根体积等指标,计算发芽率、发芽势、发芽指数、活力指数、简化活力指数、相对电导率等指标,对种子浸出液的相对电导率与甲醇浸泡时间进行线性回归分析。结果表明,幼苗各形态指标随甲醇处理时间的增加,呈逐渐下降的趋势,与浸泡时间呈负相关。与对照相比,甲醇不同浸泡时间处理的发芽率、发芽势、发芽指数、活力指数、简化活力指数分别降低2.08%~39.58%、4.49%~24.72%、0~47.5%、1.08%~58.49%、4.67%~73.08%,与浸泡时间呈负相关。种子浸出液相对电导率随老化时间延长呈逐渐升高的趋势,与浸泡时间呈正相关。

Soaking cucumber(Cucumis sativus L.) seeds in 50% methanol solution for artificial aging treatment. The indexes of dry and fresh weight, cotyl length, maximum root length and root volume of seedlings were measured, and the indexes of germination rate, germination potential, germination index, vigor index, simplified vigor index and relative conductivity were calculated. The linear regression analysis was conducted between the relative conductivity of seed leaching solution and soaking time in methanol. The results showed that the morphological indexes of seedlings decreased gradually with the increase of methanol treatment time, and showed a negative correlation with soaking time. Compared with the control, the germination rate, germination potential, germination index, vigor index and simplified vigor index were decreased by 2.08%—39.58%, 4.49%—24.72%, 0—47.5%, 1.08%—58.49%, 4.67%—73.08%, respectively, which were negatively correlated with the soaking time. The conductivity of seed leaching liquid phase increased with the aging time and was positively correlated with soaking time.