颠茄种子质量检验方法研究

Study on Seed Quality Inspection Method of Atropa belladonna

参照《GB/T 3543-1995农作物种子检验规程》,研究颠茄种子进行质量检验方法。结果表明,种子净度分析最少试验量为3 g,送检样品量不低于30 g,净度分析过20目筛;百粒法测定千粒重;水分含量测定采用种子整粒高恒温(130±2)℃烘干2 h;纵切或剥取种胚后在0.3%TTC(氯化三苯基四氮唑)溶液中30℃避光染色3 h测定生命活力;30℃恒温或20℃/30℃变温黑暗条件下于双层滤纸上进行发芽试验。

This paper refers to GB/T 3543-1995 rules for the inspection of Atropa belladonn seeds, The results showed that the sample were at least 3 g seeds, the quantity of samples to be tested shall not be less than 30 g;passed 20 mesh sieve for purity analysis;the thousand seed weight was applied for 100-seed method;the moisture of Atropa belladonn seeds was determined by intact seeds dried at high temperature of(130±2)℃ for 2 h;the vitality was determined after peeling off the embryos in 0.3% TTC(triphenyltetrazolium chloride) solution at 30 ℃ in dark for 3 h;germination rate test was carried out in dark on double-layer filter paper at 30 ℃ or 20 ℃/30 ℃.