一株强分泌刀孢蜡蚧菌的分离及对甘肃贝母腐烂病防治的研究

Study on the Isolation of A Strong Secretory Lecanicillium psalliotae and its Control of Rotten Disease of Fritillaria przewalskii

目的:从甘肃贝母中分离内生真菌,为甘肃贝母腐烂病的生物防治提供参考。方法:通过聚合酶链式反应⁃限制性内切酶长度多态性方法和ITS测序进行了川贝母及其基原种的鉴定;通过内生菌的分离鉴定、功能分析、分泌物代谢组检测、抗病性试验等手段研究其对甘肃贝母腐烂病的防治效果。结果:从甘肃贝母中分离得到一株具有强分泌能力的刀孢蜡蚧菌Lecanicillium psalliotae,其具有固氮和拮抗镰刀菌属的作用。结论:此刀孢蜡蚧菌能够有效的防治甘肃贝母腐烂病的发生,效果优于解淀粉芽孢杆菌。

Objective:To isolate the endophytic fungi from Fritillaria przewalskii,so as to provide reference for biological control of rotten disease of Fritillaria przewalskii.Methods:The identification of Fritillariae Cirrhosae Bulbus and its original species was carried out by PCR⁃restriction endonuclease length polymorphism and ITS sequencing.Its control effect on the rotten disease of Fritillaria prze⁃walskii was studied by isolation and identification of endophyte,functional analysis,secretion metabolome detection and disease resist⁃ance test.Results:A strong secretory strain of Lecanicillium psalliotae was isolated from Fritillaria przewalskii,which could fix nitrogen and antagonize Fusarium.Conclusion:Lecanicillium psalliotae can effectively prevent and control the occurrence of rotten disease of Frit⁃illaria przewalskii,and its effect is better than that of Bacillus amylolyticus.