基于转录组的多花黄精与长梗黄精代谢途径分析

Metabolic Pathway Analysis of Polygonatum cyrtonema and Polygonatum filipes based on Transcriptomic Data

目的:获得多花黄精和长梗黄精根茎转录组信息特征,解析2种黄精属植物活性成分生物合成通路的异同点。方法:以多花黄精和长梗黄精根茎为研究对象,采用Illumina HiSeq测序平台进行高通量转录组测序并进行数据分析。结果:获得154778个Unigenes,其中注释差异表达Unigenes 14135个,GO功能可分类为51个,功能富集2076条,而多糖和薯蓣皂苷代谢显著性较差;KEGG富集到114条代谢通路,前20条通路中未见多糖和薯蓣皂苷相关代谢通路;782个Unigenes参与果糖和甘露糖、半乳糖、淀粉和蔗糖代谢途径,其中228个Unigenes编码3条多糖生物合成途径中13个关键酶,但只有35个Unigenes有显著性差异;270个Unigenes参与甾醇化合物、萜类化合物骨架和单萜类生物合成等5条甾体皂苷相关代谢途径,其中64个Unigenes编码薯蓣皂苷生物合成途径9个关键酶,仅6个Unigenes有显著性差异。结论:多花黄精与长梗黄精转录组差异性大,但在多糖和薯蓣皂苷相关代谢通路中的关键酶对应的Unigenes差异较少,该结果为日后扩大黄精药材植物资源以及深入研究黄精属转录组提供参考依据。

Objective:To obtain the transcriptome informations of rhizome of Polygonatum cyrtonema and Polygonatum filipes,and to analyze the similarities and differences of biosynthesis pathways of the active components in two plants of the Polygonatum genus.Methods:Using the rhizomes of Polygonatum cyrtonema and Polygonatum filipes as research objects,Illumina HiSeq sequencing platform was used for high⁃throughput transcriptome sequencing and data analysis.Results:154778 Unigenes were obtained,of which 14135 Unigenes were differentially expressed in annotations.The GO function could be classified as 51 and 2076 were functionally en⁃riched,while the metabolism of polysaccharides and diosgenin was relatively poor;KEGG enriched 114 metabolic pathways,and polysac⁃charide and diosgenin related metabolic pathways were not found in the first 20 pathways;782 Unigenes were involved in the metabolic pathways of fructose and mannose,galactose,starch and sucrose.228 Unigenes encoded 13 key enzymes in 3 polysaccharide biosynthetic pathways,but only 35 Unigenes showed significant differences;270 Unigenes participated in 5 steroid saponin⁃related metabolic path⁃ways,including sterol compounds,terpene compounds skeleton and monoterpene biosynthesis.Among them,64 Unigenes encoded 9 key enzymes in diosgenin biosynthesis pathway,and only 6 Unigenes showed significant differences.Conclusion:There are significant differ⁃ences between the transcriptome of Polygonatum cyrtonema and Polygonatum filipes,but there is little difference in Unigenes corre⁃sponding to key enzymes in polysaccharide and diosgenin related metabolic pathways.The results will provide reference for future expan⁃sion of plant resources of Polygonati Rhizoma and further study of the transcriptome of Polygonatum genus.