基于ISSR标记的建兰种质资源遗传多样性分析

Study on Genetic Diversity of Cymbidium Ensifolium Germplasm Based on ISSR Marker

为揭示建兰种质间的亲缘关系,促进建兰种质资源的有效保护和利用。本研究利用ISSR标记分析了源于中国各地的39份建兰品种,并采用UPGMA方法进行了聚类分析。结果表明:6个ISSR引物对39份建兰品种的基因组DNA进行扩增,共扩增出64条清晰条带,其中57条为多态性条带。平均每条引物检测到的条带数为11条,多态性条带10条,多态性比率为89.88%。通过UPGMA法进行聚类分析表明供试材料的遗传相似系数在0.500~0.953之间,供试材料被分为6群集,说明ISSR标记在建兰的品种间具有丰富的多态性,能够很好地揭示建兰品种间的亲缘关系。本研究结果可为建兰种质资源品种鉴定及杂交育种等提供理论依据。

To reveal the genetic relationship between C.ensifolium germplasm,promote the effective protection and utilization of C.ensifolium germplasm resources.In this study,ISSR markers were used to analyze 39 C.ensifolium varieties originating from various parts of China,and cluster analysis was performed using UPGMA method.The results showed that 6 ISSR primers amplified the genomic DNA of 39 C.ensifolium varieties,and a total of 64 clear bands were amplified,including 57 polymorphic bands.The average number of bands detected per primer was 11,polymorphic bands were 10 and the polymorphic ratio was 89.88%.Cluster analysis by UPGMA method showed that the genetic similarity coefficient of the test materials was between 0.500–0.953,and the test materials were divided into 6 clusters,indicating that ISSR markers are rich in polymorphism in C.ensifolium varieties,which can Reveal the relationship between C.ensifolium varieties.The results of this study can provide a basis for the identification and cross breeding of C.ensifolium germplasm resources.