基于网络药理学的中药蒲公英治疗乳腺癌作用机制研究

Mechanism study of Taraxaci Herba in the treatment of breast cancer based on network pharmacology

目的运用网络药理学筛选蒲公英治疗乳腺癌的有效成分及对应靶点,构建"药物-有效成分-疾病-靶点"网络,探讨蒲公英治疗乳腺癌的作用机制。方法通过Batman TCM、PubChem及SwissTargePrediction数据库和GeneCards、OMIM数据库筛选蒲公英有效成分、疾病靶点,运用Cytoscape软件构建药物与疾病间相互作用网络,筛选蒲公英治疗乳腺癌的有效成分及靶点;基于R软件进行GO功能注释和KEGG通路富集分析,探究作用机制。结果预测得到8个蒲公英治疗乳腺癌的有效成分和42个差异基因,并推断其作用机制可能与癌症中的MicroRNA信号通路、mTOR信号通路、催乳素信号通路及RNA聚合酶Ⅱ近端启动子序列特异性DNA结合等有关,其中Akt1、PTGS2、ESR1、NFKB1、AR等基因可能为核心靶点。结论蒲公英治疗乳腺癌具有多成分、多靶点、多通路的作用特点,为阐述蒲公英治疗乳腺癌的作用机制和开展进一步研究提供依据。

Objective To explore the mechanism of Taraxaci Herba in the treatment of breast cancer by screening out the active ingredients and targets,so as to construct a herb-ingredient-disease-target network based on network pharmacology.Methods Based on Batman TCM,PubChem,SwissTargePrediction database and GeneCards and OMIM database to screen out active ingredients and corresponding targets of Taraxaci Herba by using Cytoscape software to construct the interactive network among medicine and disease to screen out the potential ingredients and targets of Taraxaci Herba in the treatment of breast cancer.Finally,the information of signaling pathway was obtained after the GO enrichment analysis and KEGG pathway analysis were performed based on R softwares,and to explore the mechanism of action.Results Expect to get 8 active ingredients,and 42 targets gene.And futher predict that the mechanism may relate to MicroRNAs in cancer signaling pathway,mTOR signaling pathway,Prolactin signaling pathway and RNA polymeraseⅡproximal promoter sequence-specific DNA signaling pathway.Among the pathways,Akt1,PTGS2,ESR1,NFKB1 and AR genes may play a crucial role.Conclusion The effect of Taraxaci Herba on breast cancer is multi-component,multi-target and multi-channel,which provides a basis for further understanding the mechanism and further research of dandelion in treating breast cancer.