纤维素三(4-甲基苯甲酸酯)手性柱对5种手性药物的拆分研究

Resolutions of Five Chiral Drugs on Cellulose Tris(4-methylbenzoate) Chiral Columns

采用C_(4)、C_(8)、C_(18)、NH_(2)修饰的大孔硅胶及纯大孔硅胶作基质,分别涂覆纤维素三(4-甲基苯甲酸酯)(CTMB)制备了5种高效液相色谱手性固定相,考察了在4种流动相下这5种手性柱对布洛芬、扁桃酸、DNB-亮氨酸、酮洛芬和氟比洛芬5种手性药物的拆分效果,为该五种手性药物的拆分提供了依据。实验结果显示,五种手性药物在五种手性柱上都有不同程度的拆分,CTMB涂覆在氨基化大孔硅胶和纯大孔硅胶对五种手性药物的整体拆分效果优于反相大孔硅胶且部分样品在正向流动相下拆分效果较差,这为这五种手性药物的实际拆分提供了参考价值。

Using C_(4), C_(8), C_(18), NH_(2)-modified macroporous silica gel and pure macroporous silica gel as substrates, five cellulose tris(4-methylbenzoate)(CTMB) chiral stationary phases for high performance liqiud chromatography were prepared. With four mobile phases, ibuprofen, mandelic acid, DNB-leucine, ketoprofen and flurbiprofen were resolved on those chiral columns. The experimental results confirm that five chiral drugs have different degrees of resolution on five chiral columns. CTMB coated on aminated macroporous silica gel and pure macroporous silica gel usually has a good resolution. This research provides a way for the actual resolution of five chiral drugs.