摘要
Background:The core concept of cell engineering is the use of synthetic biological methods to engineer and design genetically modified cells with specific functions,which has revolutionized the biotechnology industry and cell therapy.Implanted cells play an important role in the cell therapy,but the currently used implanted cells are unable to fully meet the needs of researchers and clinicians.Therefore,the construction of engineered implanted cells has become a new research area,with many groups exploring the working principles of implanted cells,allowing them to better exert their repair function.Results:Based on the existing cell engineering platforms,this paper summarizes the main types of chassis cells used in implanted cell engineering,progress in the development of gene editing tools and delivery systems,as well as strategies for the construction of engineered implanted cells.Conclusions:The rational use of synthetic biology methods to program and control the function of implanted cells with high spatiotemporal accuracy provides new ideas for the development of cell therapy,and opens up new possibilities for exploring the mechanism of implanted cell action to allow them to better exert their role in promoting the progress of repair.
基金
This work was financially supported by National Key Research and Development Project of China(No.2018YFA0900100)
the National Natural Science Foundation of China(No.31961133019)
National Natural Science Foundation of China(No.31670091)
The National Natural Science Foundation of China(No.31961133019)is part of MIX-UP,a joint NSFC and EU H2020 collaboration
In Europe,MIX-UP has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 870294.
