MDS患者血浆中3种miRNA表达水平及临床意义

Expression levels and clinical significance of three kinds of miRNAs in patients with MDS

目的:探究骨髓增生异常综合征(MDS)患者血浆mi-21、miR-155、miR-210表达水平及其临床意义。方法:选择2016年6月—2020年6月收治83例MDS患者作为研究组,同一时间体检40例健康者作为对照组,均测定血浆miR-21、miR-155、miR-210表达水平,分析2组3种miRNA差异及临床意义。研究组按照WTO分型、国际预后积分系统(IPSS)评分进行分组,比较不同WTO分型、IPSS评分患者血浆miRNA水平,分析3种血浆miRNA表达水平与WTO分型、IPSS评分之间相关性。结果:研究组患者血浆miR-21、miR-155、miR-210表达水平均显著高于对照组(P<0.05);ROC曲线显示,血浆miR-21、miR-155、miR-210用于诊断MDS患者病情AUC值分别为0.989、0.887、0.950;难治性贫血伴原始细胞增多型患者血浆miR-21、miR-155、miR-210表达水平显著高于难治性贫血型与难治性血细胞减少伴多系发育异常型(P<0.05);血浆miR-21、miR-155、miR-210表达水平与WTO分型呈正相关(r=0.406、519、0.487;P<0.05);高危组患者血浆miR-21、miR-155、miR-210表达水平显著高于低危组与中危组(P<0.05),中危组患者血浆3种miRNA表达水平显著高于低危组(P<0.05);血浆miR-21、miR-155、miR-210表达水平与IPSS评分呈正相关(r=0.612、0.814、0.553;P<0.05)。结论:血浆miR-21、miR-155以及miR-210表达水平可用于预测MDS发生,对于评估患者病情进展意义重大,可以为患者及时治疗提供临床依据。

Objective:To explore the expression levels and clinical significance of plasma miR-21,miR-155 and miR-210 in patients with myelodysplastic syndrome(MDS).Methods:A total of 83 MDS patients(study group)admitted to the hospital from June 2016 to June 2020 and 40 healthy individuals(control group)receiving physical examination during the same period were enrolled as the research objects.The expression levels of plasma miR-21,miR-155 and miR-210 were measured.Differences in the three kinds of miRNA in the two groups and their clinical significance were analyzed.Patients in the study group were grouped according to WTO classification and the International Prognostic Scoring System(IPSS)score.The plasma miRNA levels of patients with different WTO classification and different IPSS scores were compared.Meanwhile,the relationship of expression levels of the three kinds of plasma miRNAs with WTO classification and IPSS score was analyzed.Results:The expression levels of plasma miR-21,miR-155 and miR-210 in study group were significantly higher than those in control group(P<0.05).ROC curves showed that AUC values of plasma miR-21,miR-155 and miR-210 for diagnosis of MDS were 0.989,0.887 and 0.950,respectively.The expression levels of miR-21,miR-155 and miR-210 in patients with refractory anemia with excess of blasts were significantly higher than those with refractory anemia and refractory cytopenia with multilineage dysplasia(P<0.05).The expression levels of plasma miR-21,miR-155 and miR-210 were positively correlated with WTO classification(r=0.406,519,0.487.P<0.05).The expression levels of plasma miR-21,miR-155 and miR-210 in high-risk group were significantly higher than those in low-risk group and moderate-risk group(P<0.05).The expression levels of their miRNA in moderate-risk group were significantly higher than those in low-risk group(P<0.05).The expression levels of plasma miR-21,miR-155 and miR-210 were positively correlated with IPSS score(r=0.612,0.814,0.553.P<0.05).Conclusion:The expression levels of plasma miR-21,miR-155 and miR-210 can be applied to predict the occurrence of MDS,which may be of great significance for assessing the progression of patients’conditions,and can provide clinical evidence for timely treatment.