抑制PP2Acα促进胃癌进展中WTAP高表达

WTAP levels are upregulated in the progression of gastric cancer by inhibiting PP2Acα

目的:探讨蛋白磷酸酶2A催化亚基Cα(PP2Acα)的抑制是否会影响胃癌细胞的恶性表型,以及维尔姆肿瘤1相关蛋白(Wilms’tumour 1-associating protein,WTAP)在这一进展中的表达。方法:使用癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库分别比较PP2Acα编码基因(PPP2CA)、WTAP基因在胃癌组织与正常胃黏膜组织中的表达量;使用KM-plot网站(http://kmplot.com/analysis/)分析PPP2CA、WTAP与胃癌预后的关系;使用慢病毒介导的短发卡RNA(short hairpin RNA,sh RNA)在胃癌细胞中敲减PPP2CA,从而抑制PP2Acα的表达;随后提取RNA、蛋白分别进行定量逆转录PCR(quantitative reverse transcription PCR,RT-qPCR)、蛋白免疫印迹(Western blot),检测PPP2CA、WTAP的mRNA水平及蛋白的表达;最后在离体与活体层面观察抑制PP2Acα对于胃癌细胞恶性表型的影响。结果:抑制PP2Acα后,胃癌细胞发生上皮间质转化(epithelial-mesenchymal transition,EMT)形态改变,且增殖、迁移与侵袭能力均得到了增强,并伴随着WTAP蛋白及mRNA水平的显著升高。结论:抑制PP2Acɑ促进胃癌细胞的恶性表型,可能是通过上调WTAP的表达水平实现的。

Objective:This stndy aims to investigate whether the inhibition of protein phosphatase 2 A catalytic subunit Cα(PP2 Acα)affects the malignant phenotype of gastric cancer cells and the expression of Wilms tumor 1 related protein(WTAP)in this progression. Methods:The Cancer Genome Atlas(TCGA)database was used to compare the expression of PP2 Acα coding gene(PPP2 CA)and WTAP in gastric cancer and normal tissues adjacent to the cancer;the KM-plot website was used to analyze the relationship between PPP2 CA,WTAP and the prognosis of gastric cancer;lentivirus-mediated short hair Card RNA(shRNA)was used to inhibit the expression of PP2 Acα in gastric cancer cells,and then RNA and protein for PCR and WB were extracted to detect the m RNA and protein levels of PPP2 CA/PP2 Acα and WTAP,respecfively;finally,the influences of PP2 Acα inhibition on gastric cancer cells phenotype were detected in vitro and in vivo. Results:After inhibiting PP2 Acα,it was found that the proliferation,migration and invasion capabilities of gastric cancer cells were enhanced with epithelial-mesenchymal transition(EMT),accompanied by a significant increase in the protein levels of WTAP. Conclusion:Inhibition of PP2 Acɑ promotes the malignant phenotype of gastric cancer cells,which may be achieved by upregulating the levels of WTAP.