中药活性成分散沫花素对三阴性乳腺癌细胞抑制作用的初步研究

Preliminary study on the inhibitory effects of active ingredient lawsone in Chinese medicine on triple negative breast cancer

目的:探讨中草药散沫花中活性成分散沫花素(lawsone)在三阴性乳腺癌(triple negative breast cancer,TNBC)治疗中的应用前景。方法:选取2株TNBC细胞系MDA-MB-231和SUM1315作为研究对象,通过CCK-8实验、划痕实验、Transwell实验、平板克隆、EdU以及细胞周期检测等实验探讨散沫花素对乳腺癌细胞的作用。结果:MDA-MB-231和SUM1315的72 h半数抑制浓度(IC50)分别为249.87μmol/L和158.34μmol/L,散沫花素能明显抑制乳腺癌细胞迁移、侵袭及增殖,同时能延长乳腺癌细胞周期的G1期。结论:散沫花素可能通过抑制乳腺癌细胞迁移、侵袭及增殖来影响乳腺癌的发生发展。

Objective:This stndy aims to investigate the application prospect of lawsone in the treatment of triple negative breast cancer(TNBC). Methods:Two TNBC cell lines,MDA-MB-231 and SUM1315,were selected as the research subjects. The effects of lawsone on breast cancer cells were investigated by CCK-8 assay,wound-healing assay,cellular invasion assay,colony formation assay,EdU assay and cell cycle assay. Results:The 72-hour IC50 values of MDA-MB-231 and SUM1315 were 249.87 μmol/L and 158.34μmol/L,respectively. Lawsone could significantly inhibit the migration,invasion and proliferation of breast cancer cells,and prolong the G1 phase of breast cancer cell cycle. Conclusion:Lawsone may affect the occurrence and development of breast cancer by inhibiting the migration,invasion and proliferation of breast cancer cells.