冻干牛血中汞标准物质的研制

Research of the certified reference material of mecury in lyophilized bovine blood

目的研制冻干牛血中汞成分分析标准物质。方法采集牛全血加入汞标准溶液,经混匀、分装、冷冻干燥制备成两个浓度水平标准物质研制物,分别采用t检验和F检验方法对其均匀性和稳定性进行评估,采用9家实验室2种方法对标准物质进行联合定值,对制备、长期稳定性、短期稳定性、定值等过程中的不确定度进行评估,对标准物质进行赋值。结果标准物质均匀性良好,在-20℃条件下可稳定保存12个月;在≤35℃条件下运输,在7 d内量值稳定;复溶后在4℃条件下可保存5 d;量值分别为9.4、29.2μg/L,不确定度分别为±1.1、±2.3μg/L(k=2)。结论冻干牛血中汞成分分析标准物质各项指标符合技术规范要求,具有一定的应用价值。

Objective To develop a reference material for the analysis of mercury in freeze-dried bovine blood.Methods The whole blood of the bovine was collected,mercury standard solution was added,After mixing,packaging and freeze-drying.Two concentration levels of reference materials were prepared.The homogeneity and stability were evaluated by t-test and F-test methods respectively.The uncertainty in preparation,long-term stability,short-term stability and fixed value were evaluated Evaluation and assignment of reference materials.The research products were fixed value and were analyzed by two methods and fixed value in 9 laboratories and the uncertainty during development was analyzed.Results The reference material has good uniformity and can be kept stably for 12 months at-20℃;When transported at≤35℃,the quantity value is stable within 7 days;After re dissolution,it can be stored at 4℃for 5 days;The values are 9.4 respectivelyμg/L and 29.2μg/L,and the uncertainty is±1.1 respectivelyμg/L and±2.3μg/L(k=2).Conclusion The indexes of reference materials for mercury composition analysis in freeze-dried bovine blood meet the requirements of technical specifications and have certain application value.