circFARSA与 miR-671-5p在胃癌组织中的表达 及其对胃癌细胞生物学功能的影响

Expression of circFARSA and miR-671-5p in Gastric Cancer Tissues and Their Effects on Biological Functions of Gastric Cancer Cells

目的探讨circFARSA与miR-671-5p在胃癌组织中的表达及其对胃癌细胞生物学功能的影响。方法采用qRT-PCR法检测胃癌组织与癌旁组织中circFARSA、miR-671-5p的表达量;采用Pearson法分析胃癌组织中circFARSA与miR-671-5p表达量的相关性;体外培养人胃癌细胞AGS,随机分为si-NC组、si-circFARSA组、si-circFARSA+anti-miR-NC组、si-circFARSA+anti-miR-671-5p组;采用MTT实验检测细胞活力;用流式细胞仪检测细胞周期;采用划痕实验检测细胞迁移距离;采用Transwell小室实验检测细胞侵袭能力;双荧光素酶报告基因实验检测circFARSA与miR-671-5p的靶向关系。结果circFARSA在胃癌组织中呈高表达,且明显高于癌旁组织(P<0.05),癌组织中的miR-671-5p呈低表达,且明显低于癌旁组织(P<0.05);circFARSA与miR-671-5p表达呈负相关(r=-0.6374,P<0.01);与si-NC组比较,si-circFARSA组细胞活力与S期细胞比例降低(均P<0.05),迁移距离减小(P<0.05),侵袭细胞数减少(P<0.05),G0/G1期细胞比例升高(P<0.05);circFARSA可靶向调控miR-671-5p;转染si-circFARSA对细胞产生的生物学作用在anti-miR-671-5p、si-circFARSA共转染后被逆转。结论干扰circFARSA可通过调节miR-671-5p对胃癌的细胞增殖、迁移、侵袭过程发挥一定的抑制作用,同时诱导细胞周期阻滞。

Objective To explore the expression of circFARSA and miR-671-5 p in gastric cancer tissues and their effects on the biological functions of gastric cancer cells.Methods qRT-PCR was used to detect the expression of circFARSA and miR-671-5 p in gastric cancer tissues and adjacent tissues.Pearson method was used to analyze the correlation between circFARSA and miR-671-5 p expression in gastric cancer tissues.Human gastric cancer cells AGS were cultured in vitro and randomly divided into si-NC group,si-circFARSA group,si-circFARSA+anti-miR-NC group,si-circFARSA+anti-miR-671-5 p group.MTT test was used to detect cell viability.Flow cytometry was used to detect cell cycle.The scratch test was used to detect the cell migration distance.Transwell chamber experiment was used to detect cell invasion ability.The dual luciferase reporter gene experiment detects the targeting relationship between circFARSA and miR-671-5 p.Results The expression of circFARSA in gastric cancer tissues was significantly higher than that in adjacent tissues(P<0.05),and the expression of miR-671-5 p in gastric cancer tissues was significantly lower than that in adjacent tissues(P<0.05).circFARSA was negatively correlated with miR-671-5 p(r=-0.6374,P<0.01).Compared with the si-NC group,the cell viability and ratio of S phase cells in the si-circFARSA group were significantly decreased(P<0.05),migration distance was significantly decreased(P<0.05),and number of invasive cells was significantly decreased(P<0.05),while the ratio of cells in G0/G1 phase was significantly increased(P<0.05).circFARSA could target miR-671-5 p.The biological effects of si-circfarsa transfection on cells were reversed after co-transfection with anti-miR-671-5 p and si-circfarsa.Conclusion Interfering with circFARSA could inhibit the proliferation,migration and invasion of gastric cancer cells by regulating miR-671-5 p,and induce cell cycle arrest at the same time.