黄素单核苷酸的脉冲极谱法研究及其在汞电极上反应速度常数的测定

Studies on the Pulse Polarography of Flavin Mononucleotide and It’s Measurement of Rate Constant at the Mercury Electrode

本文研究了黄素单核苷酸的脉冲极谱行为,在选定的最佳条件下作了定量测定,检出限可达6.8×10^(-7)M,方法简便快速,本文还藉循环伏安法研究了黄素单核苷酸的电极反应动力学性质,测定了其在汞电极上的反应速度常数。

The differential pulse polarographic behaviour of flavin mononucleotide(FMN)and determinative conditions have been investigated.The optimum conditions for the determination of FMN are as fllows:the clectrolyte was 0.01M sodium citrate(pH 5),initial potential-0.100V(vs.Ag-AgCl),final potential-0.600V(vs·Ag-AgCl),voltage sweep rate 100mV/sec.A fair reproducibility was obtained in the above progedure and a good proportionality between the values of the peak current and FMN concentration(1.8×10^(-6)-4.7×10^(-6)M)has been observed.The decection limit is 6.8×10^(-7)M.This method provides a rapid and simple way to determination of FMN.This paper also reports that the application of cyclic voltammelry to study electrode process kinetic behavior has been established.Under the experimental conditions the rate constants for electron transfer arc determined.The values of k arc in good agreement with literature’s results.