通体结香技术产沉香的镇痛抗炎作用研究

Analgesic and anti-inflammatory effects of agarwood produced by the wholetree agarwood-inducing technique

探究通体结香技术产沉香(以下简称"通体沉香")的镇痛抗炎作用及可能机制。采用体外LPS诱导RAW264.7细胞炎症模型筛选,评价通体沉香不同温度加热熏香挥发性成分的抗炎活性;采用体内小鼠经典镇痛抗炎热板法、醋酸致小鼠扭体、二甲苯致耳肿胀及卵清蛋白(OVA)致脚趾肿胀等试验,评价通体沉香醇提物(WTAAE)预先给药处理的镇痛抗炎作用;通过检测药物处理炎症细胞培养上清中TNF-α和IL-1β的水平,探究通体沉香发挥镇痛抗炎的可能作用机制。结果显示,通体沉香加热熏香挥发性成分体外有较好抗炎活性,IC50范围为30~50μg/mL;WTAAE能显著缩短小鼠痛阈时间,降低小鼠扭体次数,且同等剂量下WTAAE作用与野生沉香醇提物(WAAE)作用相当或略优,同时缓解小鼠耳和脚趾的肿胀程度,体内有较好镇痛抗炎作用;作用机制研究显示,通体沉香挥发性成分可显著降低炎性细胞因子TNF-α和IL-1β的水平。通体沉香体内外均有较好的镇痛抗炎作用,其机制可能与抑制炎性细胞因子和致痛物质的分泌有关。

To explore the analgesic and anti-inflammatory effects and the possible mechanism of agarwood produced by the whole-tree agarwood-inducing technique(Agar-Wit),the inflammatory model of RAW264. 7 cells induced by LPS in vitro was selected to evaluate the anti-inflammatory activity of volatile components of Agar-Wit agarwood at different temperatures. The analgesic and anti-inflammatory effects of Agar-Wit agarwood alcohol extract(WTAAE)were investigated by the methods of hot plate,acetic acid induced writhing in mice,xylene induced ear swelling and OVA induced toe swelling. The possible mechanism of the analgesic and anti-inflammatory effects of WTAAE was explored by detecting the levels of TNF-α and IL-1β in the supernatant of drug-treated inflammatory cell culture. The volatile components of Agar-Wit agarwood had good anti-inflammatory activity in vitro with IC50 range of 30-50 μg/mL. The WTAAE could significantly shorten the pain threshold,reduce the number of writhing and the swelling degree of ear and toe caused by inflammatory substances,and had a good analgesic and anti-inflammatory effect in vivo. Moreover,WTAAE had the same or slightly better effect than wild agarwood alcohol extract(WAAE)at the same dose. Studies on the mechanism of analgesic and anti-inflammatory effects showed that Agar-Wit agarwood volatile components significantly reduced the levels of inflammatory cytokines TNF-α and IL-1β. Agar-Wit agarwood has good analgesic and anti-inflammatory effects,the mechanism may be related to the inhibition of inflammatory cytokines and pain-causing substances.