血清婆罗双树样基因-4、大黏着斑蛋白1、甲胎蛋白及联合检测在肝细胞癌中的诊断价值

Diagnostic value of combined detection of serum SALL4,Talinl,AFP in hepatocellular carcinoma

目的 探讨血清婆罗双树样基因-4(SALL4)、大黏着斑蛋白1(Talin1)、甲胎蛋白(AFP)及联合检测对肝细胞癌(HCC)的诊断价值。方法 选取46例肝细胞癌患者(实验组),50例肝硬化患者作为相关对照组,以同期体检的50例健康者为正常对照组。采集实验组和对照组初诊时的血样本。采用酶联免疫吸附实验检测血清SALL4、Talin1的水平,采用化学发光法检测AFP水平,并比较各组水平差异。采用受试者工作特征(ROC)曲线评估各指标单独和联合检测诊断HCC的效能。结果 实验组血清SALL4、Talin1及AFP水平均显著高于相关对照组和正常对照组(P <0. 01)。相关对照组Talin1、AFP水平均高于正常对照组(P <0. 05),SALL4水平差异无统计学意义(P> 0. 05)。血清SALL4、Talin1和AFP诊断HCC的曲线下面积分别为0. 851、0. 836、0. 796。3项联合检测诊断HCC的AUC为0. 954。结论 血清SALL4、Talin1及AFP联合诊断HCC的灵敏度与特异度较高,有利于HCC的早期诊断。

Objective To investigate the diagnostic value of the combined detection of serum SALL4,Talinl,AFP in hepatocellular carcinoma(HCC).Methods A total of 46 patients with hepatocellular carcinoma(experimental group),50 patients with liver cirrhosis were selected as the disease control group,and 50 healthy people in the same period were selected as the normal control group.Blood samples were collected from the experimental group and the disease control group at the initial diagnosis.The serum levels of SALM and Talinl were detected by enzyme-linked immunosorbent assay(ELISA),and the levels of AFP were detected by chemiluminescence method.Receiver operating characteristic(ROC)curve was used to evaluate the efficacy of each indicator in the diagnosis of HCC.Results The serum levels of SALL4,Talinl and AFP in the experimental group were significantly higher than those in the disease control group and the normal control group(P<0.01).The serum levels of Talinl and AFP in the disease control group were significantly higher than those in the normal control group(P<0.05),but there was no statistical significance on the difference in SALL4 level(P>0.05).The area under the curve of serum SALM,Talinl and AFP in the diagnosis of HCC was 0.851,0.836 and 0.796,respectively.The AUC of the three combined tests was 0.954.Conclusion The sensitivity and specificity of serum SALLA,Talinl and AFP in the diagnosis of HCC are high,which is conducive to the early diagnosis of HCC.