HPLC-DAD-ELSD法测定丹膝颗粒中8种成分的含量

Quantitative Determination of Eight Constituents in Danxi Granules by HPLC-DAD-ELSD

目的:建立HPLC-DAD-ELSD法测定丹膝颗粒中8种成分的含量。方法:色谱柱:CAPCELL PAK C18 MGⅡ(4.6 mm×250 mm,5μm);流动相:乙腈-0.05%甲酸溶液,梯度洗脱;流速:0.8 ml·min^(-1);柱温:35℃;进样量:10μl。结果:没食子酸、栀子苷、丹酚酸B、淫羊藿苷、丹皮酚、丹参酮ⅡA、人参皂苷Ro、橙黄决明素进样量分别在0.10~1.25μg、0.20~2.50μg、0.40~5.00μg、0.10~1.25μg、0.20~2.50μg、0.03~0.38μg、0.03~0.38μg、0.03~0.38μg(r为0.9989~0.9999)范围内与峰面积呈良好线性关系,平均回收率分别为99.5%,100.1%,98.0%,101.2%,98.9%,99.3%,98.5%,97.8%(RSD<2.0%,n=6)。结论:该方法操作简便、结果可靠,重复性好,可用于丹膝颗粒的质量控制。

Objective:To establish an HPLC-DAD-ELSD method for the quantitative determination of eight constituents in Danxi granules.Methods:A CAPCELL Pak C18 MGⅡcolumn(4.6 mm×250 mm,5μm)was used,and acetonitrile-0.05%methanoic acid solution was used as the mobile phase with gradient elution.The flow rate was 0.8 ml·min^(-1),and the column temperature was set at 35℃with the injection volume 10μl.Results:Gallic acid,geniposide,salvianolic acid B,icariin,tanshinoneⅡA,ginsenoside Ro and aurantio-obtusin showed good linear relationship within the range of 0.10-1.25μg,0.20-2.50μg,0.40-5.00μg,0.10-1.25μg,0.20-2.50μg,0.03-0.38μg,0.03-0.38μg and 0.03-0.38μg(r=0.9989-0.9999),respectively.The average recovery was 99.5%,100.1%,98.0%,101.2%,98.9%,99.3%,98.5%and 97.8%(RSD<2.0%,n=6),respectively.Conclusion:The method is simple,accurate,stable and reliable.It can be used for the quality control of Danxi granules.