基于网络药理学的健脾祛痰和血方治疗冠心病的分子机制研究

Molecular mechanisms of spleen-invigorating,phlegm-eliminating and blood formulation for coronary heart disease based on network pharmacology

[目的]以网络药理学作为研究手段,研究健脾祛痰和血方治疗冠心病的作用靶点及信号通路,揭示健脾祛痰和血方治疗稳定型心绞痛的分子机制。[方法]采用中药系统药理数据库和分析平台(TCMSP)、中医药信息数据库(TCMID)、中药潜在药物靶标数据库(TCM-PTD)检索该方剂对应的药物化合物,通过ChemMapper数据库预测药物靶点,通过TTD、disgenet、human phenotype ontology数据库进行检索,获得冠心病相关靶点,以venny图得到疾病及药物的共同靶点,应用功能蛋白结合网络string平台构建蛋白互作模型,采用cytoscape的cluego插件富集分析,得到该方剂治疗冠心病相关靶标基因及代谢通路。[结果]获得药物靶点103个,疾病靶点980个,健脾祛痰和血方42个药物靶点与冠心病相关,通过筛选得到主要药物成分9个,最终预测到25个相关的信号通路,在此基础上构建了主要药物成分-病药共性靶点-信号通路共表达网络。[结论]健脾祛痰和血方可能通过多靶点、多途径干预冠心病稳定型心绞痛,为后续基础及进一步临床研究提供思路和线索。

[Objective]To study the target and signaling pathway of invigorating spleen and expelling phlegm and blood formulation for coronary heart disease by means of network pharmacology,and to reveal the molecular mechanism of invigorating spleen and expelling phlegm and blood formulation for stable angina pectoris.[Methods]TCMSP,TCMID and TCM-PTD databases were used to retrieve the corresponding drug compounds.The drug targets were predicted by ChemMapper database.The related targets of coronary heart disease were retrieved by TTD,disgenet and human Phenotype Ontology database.The common targets of diseases and drugs were obtained by Venny diagram.The protein interaction model was constructed on the platform of functional protein binding network string.The target genes and metabolic pathways related to the treatment of coronary heart disease were obtained by enrichment analysis of the cluego plug-in of cytoscape.[Results]A total of 103 drug targets,980 disease targets,42 drug targets of invigorating spleen,eliminating phlegm and blood formulationwere related to coronary heart disease.Nine main drug components were screened and 25 related signaling pathways were predicted.On this basis,a common drug target-signaling pathway co-expression network was constructed.[Conclusion]Spleen-invigorating,phlegm-eliminating and blood formulation may intervene stable angina pectoris of coronary heart disease through multi-target and multi-channel,and provide ideas and clues for follow-up basic and further clinical research.