摘要
茚三酮与赖氨酸的显色反应已广泛用于各种样品的测定,但高灵敏的氨基酸荧光测定法的研究还很不够。尽管茚三酮与某些氨基酸的荧光反应已有报道,但反应条件苛刻,反应时间长达60~120min,对赖氨酸的专一测定法尚未见报道。
This paper describes a new method for fluorometric determination of Lysine(Lys)with ninhydrin.The quantum yield of reaction is 0.51.The 1Rand NMR spectra of product formed were determined.The mechanism of reactionwas discussed,preliminary,too.The other amino acid interference was discoussed.The method is simple and rapid.The Lysine in sample was determined withsatisfactory and precision.
作者
王怀公
姬洪涛
Wang Huaigong;Ji Hongtao(Department of Chemistry Lanzhou University,Lanzhou)
出处
《高等学校化学学报》
SCIE
EI
CAS
1988年第7期732-734,共3页
Chemical Journal of Chinese Universities
基金
中国科学院科学基金。
关键词
荧光反应
茚三酮
赖氨酸
Fluorescence reaction
Ninhydrin
Lysine
